qPCR Application Workshops

All three workshops are hosted by TATAA Biocenter (www.TATAA.com)
The qPCR workshop laboratories and seminar rooms are very close to the central lecture hall.

The workshops are aimed at giving participants a deep and objective understanding of real-time quantitative PCR, biostatistics, expression profiling, and its applications. The courses are intended for academic or industrial persons considering working with qPCR or scientists currently working with qPCR seeking a deeper understanding.

different 2-day workshops will be held in parallel at   31st March - 1st April 2011
Workshop topics:
All workshops offer extensive hands-on training by qPCR experts from the TATAA team.
Leading probe technologies will be demonstrated, common and emerging instrument platforms will be available and advanced primer and probe design programs will be used. Hands-on training will be provided on quantitative gene expression, including design and optimization of both RT and qPCR protocols.
Participating companies are asked to support the workshops with PCR cyclers, qPCR kits, consumables, centrifuges, pipettes etc….
Please contact Martina Reiter Martina.Reiter@bioEPS.com  for information and details.

In the qPCR data analysis workshop  the data conversion, normalisation procedure, biostatistical calculations and the expression profiling will be done with the newest GenEx software by MultiD.
You can download a free trial version from our webpage => Genex.gene-quantification.info

or directly here GenEx 5 download as ZIP and RAR version =>  

logo MultiD

The courses cover all aspects in qPCR and is held during 2-days. Each course is app. 50% hands-on and is limited to app. 25 participants, resulting in very interactive teaching and everybody given the opportunity to try the instrumentation.
After the course participants will be able to plan and perform qPCR experiments themselves, as well as interpret and analyze data. Detailed course material and full catering (lunch, coffee, soft drinks and snacks) are included in the course fee.

Course starts at   9:00
Course ends around  17:00

Internet based REGISTRATION platform  =>  http://registration.qPCR2011.net

Basis Module - real-time PCR Application Workshop  (2-days):

This introductory course consists of a theoretical part and a practical part where participants get to do qPCR experiments by themselves under experienced supervision. The course contains:

Day 1 - hands on workshop:
•    Basic PCR theory
•    The theory of real-time PCR
•    Applications and possibilities of qPCR. Comparison of qPCR with regular PCR.
•    Review of currently available detection technologies (SYBR Green I, hydrolysis probes, Molecular Beacons...etc)
•    Different instrument platforms and their typical uses
•    Primer design
•    The problem of primer-dimer formation and how to minimize them
•    Probe design
•    Experimental design and optimization
•    Basic data handling and analysis

Day 2 - hands on workshop:
•    Introduction to quantification principles
•    Quantification strategies, uses and limitations
•    Calculations using different relative quantification methods
•    Strategies for normalization of qPCR data
•    In situ calibration for compensation of inhibition in samples
•    Absolute quantification 

qPCR data analysis - Biostatistics & Expression Profiling  (2-days):

This course will teach you how appropriate statistics should be selected and applied correctly to get the most out of your qPCR data. The course mixes theoretical lectures with computer based exercises.   Please bring your own Laptop to the course!

Day 1- Statistical analysis of real-time PCR data
•    Basic principles of statistics
•    Advanced principles of statistics
•    Statistical tests
•    Ability to detect a difference

Day 2- Gene expression profiling with real-time PCR
•    Multiplate measurements
•    Standard curves and absolute quantification
•    Experimental design, Selecting reference genes
•    Relative quantification,Comparison of groups
•    Expression profiling

MIQE guidelines (1 day)   &   Practical primer design (1-day)

Day 1 - The MIQE guidelines – How to improve your qPCR quality
This is a theoretical course that will take you through the MIQE guidelines. The MIQE guidelines (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) have been developed to ensure the integrity of the scientific literature, promote consistency between laboratories and increase experimental transparency. The course will explain the different steps that are necessary to fulfill to ensure good qPCR quality and show you how it can be done. The course includes:
•    Introduction to the MIQE guidelines
•    Sample preparation
•    Nucleic acid extraction and quality control
•    Reverse Transcription
•    Primer and probe design
•    Optimization and validation of qPCR
•    Normalization
•    qPCR data analysis

Day 2 - Practical primer design
This is an extensive primer design course that will teach you how to design your own assays. It will teach you the theory of primer and probe design as well as how to optimize your reactions. The course will also let you do design of your own primers using primer design softwares under experienced supervision.

    qPCR workshop sponsors:

logo Bio-Rad

Any questions =>  Please contact Martina Reiter Martina.Reiter@bioEPS.com