All three
workshops are hosted by TATAA
Biocenter (www.TATAA.com)
The
qPCR
workshop
laboratories and seminar rooms are very close to
the central lecture hall.
The workshops are
aimed at giving participants a deep and objective understanding of
real-time quantitative PCR, biostatistics, expression profiling, and
its applications. The courses are
intended for academic or industrial persons considering working with
qPCR or scientists
currently working with qPCR seeking a deeper understanding.
Three
different
2-day
workshops
will be held in parallel at 31st March - 1st
April 2011
All
workshops
offer extensive hands-on training by qPCR experts from the TATAA team.
Leading
probe technologies will be
demonstrated,
common and emerging instrument platforms will be available and advanced
primer and probe design programs will be used. Hands-on training will
be provided on quantitative gene expression, including design and
optimization of both RT and qPCR protocols.
Participating companies are asked to support the workshops with PCR
cyclers, qPCR kits, consumables, centrifuges, pipettes etc….
Please
contact Martina Reiter Martina.Reiter@bioEPS.com
for
information
and details.
In the qPCR
data
analysis workshop the data conversion,
normalisation procedure, biostatistical calculations
and the expression profiling will be done with the newest GenEx
software by MultiD.
You can download a free trial version
from our webpage => Genex.gene-quantification.info
or directly here GenEx
5 download as ZIP
and RAR version =>
The
courses cover all aspects in qPCR and is held during 2-days. Each
course is app. 50%
hands-on and is limited to app. 25
participants,
resulting in very interactive teaching and everybody given the
opportunity to try the instrumentation.
After
the course participants will be able to plan and perform qPCR
experiments themselves, as well as interpret and analyze data. Detailed
course
material and full catering (lunch, coffee, soft drinks and
snacks) are included in the course fee.
This introductory course consists of a
theoretical part and a practical part where participants get to do qPCR
experiments by themselves under experienced supervision. The course
contains:
Day
1 - hands on workshop:
• Basic PCR theory
• The theory of real-time PCR
• Applications and possibilities of qPCR. Comparison
of qPCR with regular PCR.
• Review of currently available detection
technologies (SYBR Green I, hydrolysis probes, Molecular Beacons...etc)
• Different instrument platforms and their typical
uses
• Primer design
• The problem of primer-dimer formation and how to
minimize them
• Probe design
• Experimental design and optimization
• Basic data handling and analysis
Day
2 - hands on workshop:
• Introduction to quantification principles
• Quantification strategies, uses and limitations
• Calculations using different relative
quantification methods
• Strategies for normalization of qPCR data
• In situ calibration for compensation of inhibition
in samples
• Absolute quantification
qPCR data
analysis - Biostatistics
& Expression Profiling
(2-days):
This course will teach you how appropriate statistics
should be selected and applied correctly to get the most out of your
qPCR data. The course mixes theoretical lectures with computer based
exercises. Please
bring
your
own Laptop
to the course!
Day
1- Statistical analysis of real-time PCR data
• Basic principles of statistics
• Advanced principles of statistics
• Statistical tests
• Ability to detect a difference
Day
2- Gene expression profiling with real-time PCR
• Multiplate measurements
• Standard curves and absolute quantification
• Experimental design, Selecting reference genes
• Relative quantification,Comparison of groups
• Expression profiling
MIQE
guidelines (1 day) & Practical
primer design (1-day)
Day 1 -
The MIQE guidelines – How to improve your qPCR quality
This is a theoretical course that will take you
through the MIQE guidelines. The MIQE guidelines (Minimum Information
for Publication of Quantitative Real-Time PCR Experiments) have been
developed to ensure the integrity of the scientific literature, promote
consistency between laboratories and increase experimental
transparency. The course will explain the different steps that are
necessary to fulfill to ensure good qPCR quality and show you how it
can be done. The course includes:
• Introduction to the MIQE guidelines
• Sample preparation
• Nucleic acid extraction and quality control
• Reverse Transcription
• Primer and probe design
• Optimization and validation of qPCR
• Normalization
• qPCR data analysis
Day 2 - Practical primer
design This is an extensive primer design course
that will teach you how to design your own assays. It will teach you
the theory of primer and probe design as well as how to optimize your
reactions. The course will also let you do design of your own primers
using primer design softwares under experienced supervision.
