qPCR 2011 Symposium Talks

Keynote speakers: preliminary titles:

MIGE guidelines

Stephen A. Bustin
Professor of Molecular Science, Institute of Cell and Molecular Science, Queen Mary's School of Medicine and Dentistry, University of London, UK

Stephen Bustin obtained his PhD from Trinity College, University of Dublin in molecular genetics in 1983. Since 1989 he has worked at the Royal London Hospital, aiming to apply his research in a more direct, practical setting. Following promotion to Senior Lecturer (1995) and Reader in Molecular Medicine (2002) he was awarded a personal chair by the University of London in 2004. He was appointed as a visiting Professor of Molecular Biology by the University of Middlesex in 2006. His main area of research is into bowel-associated pathologies, especially colorectal cancer and, more recently, Clostridium difficile-associated disease. He has a special interest in molecular technologies and his laboratory operates at the forefront of technological development in nucleic acid quantification, where he is an internationally acknowledged leader. He has published numerous peer-reviewed papers and reviews and is the editor of the “A-Z of quantitative PCR”, the leading textbook for this technology. He is on the editorial boards of several journals and has given numerous presentations at scientific conferences around the world. He has organized and co-organised many qPCR meetings in the UK, Europe and the US.

MIQE compliance and impact factor-which results to trust?

digital PCR
Dennis YM Lo
Department of Chemical Pathology,  The Chinese University of Hong Kong, China

Professor Dennis Lo Yuk-ming is the Dr. Li Ka Shing Professor of Medicine and Professor of Chemical Pathology at The Chinese University of Hong Kong. He is also the Associate
ResearchDean of the Faculty of Medicine and the Director of the Li Ka Shing Institute of Health Sciences at CUHK. Professor Dennis Lo’s main research interest is the study of cell-free DNA and RNA molecules which exist in the plasma of human subjects. He discovered in 1997 that an unborn fetus will release its DNA into the plasma of a pregnant woman. This finding has opened up a new approach of non-invasive prenatal diagnosis. He has also applied a similar strategy to the detection of cancers which are common in Hong Kong, including nasopharyngeal cancer and liver cancer.

Noninvasive prenatal diagnosis using fetal DNA in maternal plasma:  from digital PCR to next-generation sequencing

single-cell qPCR
Mikael Kubista
Professor of Biotechnology, TATAA Biocenter Sweden, Göteborg, Sweden

Mikael Kubista is head of department of gene expression at the institute of Molecular Genetics, Czech Academy of Sciences, and founder of TATAA Biocenter AB. Inventor of several of the technologies used at TATAA Biocenter. Founder also of LightUp Technologies AB and MultiD Analysis.

25 years of PCR - from idea to subcellular expression profiling

Next Generation Sequencing

Yu-Hui Rogers
Vice President, Core Technology Development and Services, J. Craig Venter Institute, San Diego, USA

Yu-Hui Rogers is a recognized leader in the field of designing and deploying large-scale DNA sequencing projects and pipelines, Yu-Hui C. Rogers joined the Venter Institute in 2002. She currently serves as the Vice President of Core Technology Development and Services at the J. Craig Venter Institute. Ms. Roger’s areas of expertise include development, implementation, and management of DNA sequencing facilities and nucleic acid technology development. Before joining the Venter Institute, she was the Manager of Sequencing Research and Development at Celera Genomics. She was instrumental in the development and implementation of the Celera high-throughput sequencing pipeline that allowed the human genome sequence to be completed in 14 months. In addition, she was responsible for implementing and managing a forensic resequencing pipeline at Celera. This pipeline was specifically set up to perform the mtDNA resequencing on the World Trade Center (WTC) victim and reference samples for the purpose of identifying the WTC victims. Prior to joining Celera, she was a scientist at Molecular Tool, Inc. At Molecular Tool, she was involved in the developed of several novel methods for nucleic acid sequence detection and analysis.

Research Overview & Application of Next Generation Sequencing Technologies at JCVI


Jo Vandesompele
Center for Medical Genetics Ghent, Ghent University Hospital, Ghent, Belgium

Jo Vandesompele is a Professor in bioinformatics and biocomputing at the Center for Medical Genetics, Ghent University since 2007. He is author of various pioneering publications in the area of real-time PCR. Together with Jan Hellemans he developed advanced and universally applicable quantification methods for automated and accurate qPCR data analysis. He is also the co-founder of Biogazelle, the real-time PCR data-analysis company.

Measurable impact of RNA quality on gene expression results from quantitative PCR

Invited academic speakers:

preliminary titles:

Single-cell qPCR

Anders Stahlberg
PhD, Stem Cell Center, Lund University, Lund, Sweden

Understanding Tumor Heterogeneity Using Single-Cell Gene Expression Profiling
microRNA analysis

Jan Helemanns
PhD, Center for Medical Genetics Ghent, Ghent University Hospital
, Ghent, Belgium
Biogazelle NV, Kruishoutemstraat 57, Zulte, Belgium

Development of an ultra-high throughput long non-coding RNA qPCR screening system

Robert Löwe
CEO, Genewake Pharmacogenomics,  München-Neuried, Germany

Quantification of specific mutation with HRM for regions of interest
NGS & qRT-PCR application

Michael W. Pfaffl
Physiology, Center of Life Science, Weihenstephan, Technical University of Munich, Freising, Germany
The use of qRT-PCR and high throughput transcriptomics for biomarker development

digital PCR
Frank McCaughan
Medical Research Council, Laboratory of Molecular Biology, Cambridge Cancer Center, Cambridge, UK

Digital PCR - getting the most out of difficult clinical sample

Bertram Brenig
Institute of Veterinary Medicine, Dept. of Molecular Biology of Livestock, Georg August University Göttingen, Germany

Analysis of circulating nucleic acids (CNA) using different NGS technologies

High Throughput

Filip Pattyn
PhD, Center for Medical Genetics Ghent, Ghent University Hospital
, Ghent, Belgium

PrimerXL: high-throughput assay design for qPCR and amplicon based NGS

MIQE & QM session
Afif Abdel Nour  &  Michael W. Pfaffl
Associate Professor in Molecular Biotechnology, Institut Polytechnique LaSalle Beauvais, France   & 
Professor in Molecular Physiology, TUM, Freising, Germany

"Stay in touch while on the bench"  MIQE qPCR

bioinformatics & optimisation

David Kennard
CEO, AzurePCR, London, UK   &   Ramat Gan, Israel

Is normalisation neccessary?  A novel method for fully automated analysis of qPCR using raw cycler output data

Single-cell qPCR / pre-PCR

Karl H. Hasenstein
Professor in Biology, University of Louisiana, Lafayette, LA, USA

Solid Phase Gene Extraction – Sampling of mRNA from living systems
high-throughput PCR / NGS

Kevin Knudtson 
Director, DNA Facility at University of Iowa, Iowa City, USA

Using Nanoscale PCR and NGS Technologies to Detect Rare Sequence Variants in a Core Setting

bioinformatics Philip Zimmermann
PhD, Group Leader - Genevestigator Project at ETH Zurich, ETH-Zentrum, Institut für Pflanzenwissenschaften, Zürich, Switzerland

Interpretation requires context - making sense out of gene lists and networks.
Swanhild Meyer
Physiology, Center of Life Science, Weihenstephan, Technical University of Munich, Freising, Germany

Normalization strategies and miRNA profiling data

NGS Andreas Nitsche
Head of Konsiliarlaboratorium für Pockenviren, Robert Koch-Institut, Berlin, Germany

Deep sequencing as diagnostic tool for highly pathogenic viruses

bioinformatics & optimisation
Andreas Untergasser
Guest Scientist, Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ-ZMBH Alliance, Heidelberg, Germany

Primer3:  Improvements for the design of qPCR primers
Single-cell qPCR Philip Day
Reader in Quantitative Analytical Genomics, Manchester University, Director of Quantitative Molecular Medicine and Principal Investigator at the Manchester Interdisciplinary Biocentre, UK

Single cell analyses of bcr-abl transcripts in leukemia reveals distinct sub-populations
Ales Tichopad
Staff Scientist, Technical University of Munich, Freising, Germany

Stratified error in the qPCR assays from the statistical point of view
RDML session

Jan Helemanns  &  Andreas Untergasser
RDML consortium

RDML - structured language for real-time quantitative PCR data
RDML - state of the art & open discussion on Wednesday afternoon

MIQE session

Stephen Bustin,  Mikael Kubista,  Jo Vandesompele  &  Michael W. Pfaffl
MIQE authors

The MIQE guidelines - minimum information for publication of quantitative real-time PCR experiments
MIQE - state of the art & open discussion
on Wednesday afternoon

Industrial sponsored speakers:

preliminary titles:

Lead Sponsor:

Sabine Lohmann
Roche Diagnostics GmbH, Nonnenwald, Penzberg, Germany

Customized, function-tested RealTime ready assays for gene expression analysis in biomarker reasearch and early drug development

NGS Paolo Piazza
Genomic Services of the Wellcome Trust Center of Human Genomics in Oxford
invited by Agilent Technologies
Improved library quantification for High Throughput Sequencing at the Wellcome Trust of Human Genetics Oxford
Barbara D'haene
Scientist, Biogazelle NV, Kruishoutemstraat 57, Zulte, Belgium
invited by Roche Diagnostics
Convenient and reliable gene expression profiling using RealTime ready Focus Panels in combination with sound data-analysis using qbasePLUS

Diagnostics in single-cells
Jan Detmers
CEO, Chimera Biotec, Biomedizinzentrum, Technologie-Park Dortmund

invited by Agilent Technologies
Quantitative Immuno-PCR based on Imperacer: Technology, Platform & Analytical Applications

Gold Sponsors:
single-cell qPCR
Arjun Raj
Principal Investigator, Assistant Professor, Penn Bioengineering,  Skirkanich Hall, Room 240, Philadelphia, USA

invited by Biosearch Technologies
Single Molecule RNA FISH: novel, simple, and accurate quantitative applications for gene expression analysis

Ditte Andreasen
Senior Scientist , Exiqon, DK-2950 Vedbaek, Denmark

Towards blood-based cancer screening using LNA™-enhanced miRNA qPCR

Rygus Thomas
Life Technologies GmbH, Applied Biosystems Division, Germany

Qualitative and Quantitative Analysis of RNA by SOLiD Next Generation Sequencing
from Analog to Digital Expression

Jim White
Scientist, Nanostring, Banstead, United Kingdom

Digital gene expression, miRNA and CNV - a bridge to the Clinic
Frank Bizouarn
Bio-Rad Laboratories, Gene Expression Division, Hercules, CA and 2Bio-Rad Laboratories, Germany

Quantitative Assessment of Chromatin Structure: Analysis of Epigenetically Regulated Gene Promoters Using Real-time PCR

Pre-PCR / CNA Martin Horlitz
Senior Scientists von R&D, Qiagen, Hilden, Germany

Isolation and Analysis of Circulating Nucleic Acids: Technical Advances
digital PCR
Elena Grigorenko
Senior Scientist, Life Technologies, Inc, United States of America

Digital PCR or Finding The Needle In A Haystack
single-cell qPCR Ken Livak
Fluidigm, South San Francisco, CA, USA

Uncovering the Diversity of Individual Cells:  Gene Expression Profiling with the BioMark System
Jaakko Kurkela
Research and Development Manager, Thermo Fisher Scientific, ABgene House, Blenheim Road, Epsom, Surrey, KT19 9AP, England

Improved Accuracy of Relative Gene Expression
Scott Rose
Director of Molecular Biology Applications,
IDT - Integrated DNA Technologies

Designing Genome-wide qPCR Assays with ZEN™ Double-Quenched Probes
microRNA / RNAi Nikos Hontzeas
Global Product Manager, Functional Genomics,
Sigma Life Science, Sigma-Aldrich Corporation, St. Louis, MO, USA

microRNA target validation:  MISSION 3'UTR Lenti GoClones  and Human MicroRNA Mimics

All scientific contributions will be published in the  qPCR 2011 Symposium Proceedings  ISBN 978-3000338403

Any changes ???   =>   please contact the scientific organizer Michael W. Pfaffl via    qPCR2011@wzw.tum.de