TALK
& POSTER CALL for qPCR 2011 Event
PDF version
qPCR
2011 - Talk and
Poster sessions
Main
topic: Molecular
diagnostics in single-cells
Single-cell sampling, circulating tumor cells
(CTS), pre-amplification
techniques, laser micro dissection, sub-cellular PCR,
micro-manipulation of
cell clusters, cellular micro injection, FACS spotting, single-cell
handling, …
Markers in diagnostic, prognostic, and therapeutic,
markers on DNA, RNA, microRNA level, disease
markers, tissue specific markers, cancer markers, stem-cells markers,
differentiation
markers, methylation markers diagnostic
quantification methods, epigenetics, SNP analysis, high resolution melt
(HRM) applications, ...
Main topic: High
throughput
analysis
in
qPCR & NGS (Next
Generation Sequencing)
Next generation Sequencing (NGS); high throughput and
gene
expression profiling platforms, 384 – 1536 well applications and more,
NGS application
on DNA, mRNA and microRNA level, comparison of NGS with conventional
high
throughput qPCR, qPCR robotics; high throughput SNP application,
real-time
RT-PCR arrays (mRNA and microRNA), quantitative multiplexing …
MIQE and QM
strategies in qPCR
The MIQE guidelines: minimum
information for publication of quantitative real-time PCR experiments.
Following these guidelines will encourage better experimental practice,
allowing more reliable and unequivocal interpretation of qPCR results.
QM
strategies in real-time PCR to guarantee better and more valid
results...
small RNAs qPCR -
microRNA and siRNA
Applications
RNAi mechanism, microRNA
extraction, qRT-PCR technologies to detect microRNA, siRNA
applications
in combination with qRT-PCR, microRNA
targets and
microRNA precursors, new siRNA manipulation and microRNA
technologies, .....
Digital PCR
& Nano-fluidics
Digital PCR (dPCR) can be used to directly quantify and
clonally amplify
nucleic acids including DNA, cDNA, mRNA or microRNA. It allows a more
reliable
collection and sensitive measurement of nucleic acid amounts,
applications in copy
number variants, point mutations, and molecular diagnostics; …
Pre-analytical Steps
Pre-amplification, sampling technologies, DNA /
RNA purification, extraction
efficiency, DNA
/ mRNA / microRNA quality control, Reverse Transcription, RT quality
control,
external references, ...
qPCR BioStatistics
& BioInformatics
Software applications, data mining, calculation
of
relative expression, primer and probe design on mRNA and microRNA
level, real-time
PCR efficiency determination, mathematical modelling, multivariate
expression
profiling raw data analysis, statistics in
real-time PCR, data management, multi-way
expression profiling, multiple regression analysis, 3D data
visualization.......
- qBASE - data
analysis lunch seminar
- GENEX - data
analysis lunch seminar
- MIQE - state
of the art & open discussion
- RDML - state
of the art & open discussion
Internet based
REGISTRATION and ABSTRACT submission platform => http://registration.qPCR2011.net
