last update 15th June 2010
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Follow our next qPCR & NGS Event in March 2013 with the focus - Next Generation Thinking in Molecular Diagnostics

Dear researcher,

The great international interest of the previous International qPCR Events from 2004 to 2009 with up to 600 participants from over 40 countries, and 32 international companies in the Exhibition motivates repeating the success next year in Vienna - qPCR 2010 Vienna international symposium 7th – 9th April 2010. The focus of the qPCR 2010 Event will be “The ongoing evolution of qPCR” representing all new and emerging techniques, applications and data analysis methods. HRM, microRNA, CNV, single-cell qPCR, digital PCR, and analysis of circulating nucleic acids will be in the focus of the conference.

The talks by the plenary lecturer, selected invited academic and industrial speakers
will be published in METHODS special qPCR Vol 50 issue 4  (April 2010)  with the title “The ongoing evolution of qPCR”.   At the meeting all participants will get  a free hard cover of the issue  =>  please view the TOC and  HTML or PDF versions

Why Vienna?

The Vienna region is Austria’s largest life sciences location. The Austrian government and the City of Vienna set up a biotechnology network (LISA VR with the aim of having one central body to provide support and advice to start-ups and high-tech companies in the biotec field. Currently 175 life science companies employ 11,000 people in the greater Vienna region. In addition an estimated 4,500 academic researchers work in the life science sector. Vienna is the economic and biotechnology hub for Central and Eastern Europe and has become an innovative center for the life science in recent years.

The event is structured in two parts:
1)  an international qPCR Symposium taking place April 7 - 9, including various talk sessions in the big lecture hall fitting 350 persons, 
2)  a parallel qPCR Industrial Exhibition with 40 companies in the Aula and the Basement of the Juridicum (Juridicum der Universität Wien) directly in the city center of Vienna.

The scientific organization is managed by international well-known scientists in the field of qPCR:

Stephen Bustin
Mikael Kubista 
Christine Mannhalter
Jo Vandesompele

Michael W. Pfaffl
Prof. of Molecular Science, School of Medicine, London, UK
Prof. of Biotechnology, TATAA Biocenter, Sweden
Prof. for Molecular Diagnostics in Clinical Chemistry, Med. University Vienna, Austria
Prof. at the Center of Medical Genetics, University of Ghent, Belgium
Senior Scientist and Reader in Physiology, Technical University of Munich, Weihenstephan, Germany (scientific coordination)

qPCR Symposium topic  - “The ongoing evolution of qPCR”

The symposium focus will be on 40 lectures presented by international recognised experts in their application fields. The emphasis will be on unbiased, didactic information exchange. One third of the talks will be presented by invited speakers, one third of the speakers will be selected from the submitted abstracts and one third will be qPCR company representatives with their newest qPCR technologies.

The focus of the qPCR 2010 Event will be  “The ongoing evolution of qPCR”  representing all new and emerging techniques, applications and data analysis methods:

Symposium sessions & preliminary titles

MIQE and QM strategies in qPCR
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Following these guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of qPCR results. QM strategies in real-time PCR to guarantee better and more valid results.  
Prof. Stephen Bustin,  “The MIQE Guidelines:  Minimum Information for Publication of Quantitative Real-Time PCR Experiments”

Prof. Christine Mannhalter,  "Standardization efforts of qPCR: Example BCR-ABL Translocation"
Dr. Roderic Fürst,   "Optical artefacts: Cause, Consequence, Correction"  IT-IS Lifescience
Dr. Mark Behlke,   "Hydrolysis probes with improved quenching and performance"  INTEGRATED DNA TECHNOLOGIES
Dr. Alexander Rácz,  "Advanced qPCR - one step ahead with next generation dyes."   Eurofins MWG Operon
Dr. Francisco Bizouarn,   "A Practical Approach to Assay Design for qPCR - Overcoming Difficult Assays, Designs and Optimization while Conforming to the MIQE Guidelines"   Bio-Rad
Dr. Ian Kavanagh,   "Detection of Related AKT Family Members using Solaris qPCR Gene Expression Assays"   Thermo Scientific

High throughput quantitative PCR – digital PCR
384 well applications, new high throughput platforms, droplet PCR, qPCR robotics, digital PCR, gene expression real-time RT-PCR arrays (mRNA and microRNA), quantitative multiplexing, …
Prof. Mikael Kubista, “Digital PCR and intra-cellular expression profiling” TATAA BIOCENTER
Dr. Philip Day, “High throughput droplet PCR”
Dr. Gudrun Tellmann, "High-Throughput Gene Expression Analysis Using the LightCycler Platform" ROCHE
Dr. Leila Smith, "Fluidigm’s BioMark System for Gene Expression, Genotyping, Digital PCR and other sample preparation applications using nanoliter reaction volumes" FLUIDIGM
Dr. Marjaana Pussila, "Western-type diet affects the expression of genes known to be involved in human colorectal carcinogenesis in histologically normal mucosa of ApcMin/+ mice" invited by LONZA
Dr. Simone Kreth, "Prognostic Impact of Gene Expression Analyses in Human Glioblastoma" invited by ROCHE
Dr. Raza Ahmed, "Rapid Quantification of DNA libraries for Next Generation Sequencing"  Agilent Technologies
Dr. Ian Kavanagh, "Detection of Related AKT Family Members using Solaris qPCR Gene Expression Assays"  Thermo Scientific

HRM – High Resolution Melting - Epigenetics
SNP analysis, HRM = high resolution melt applications, Epigenetics, methylation markers, HRM platform comparison, etc …
Prof. Carl Wittwer, "High Resolution Melting Analysis"
Prof. Claudio Orlando, “High Resolution Melting Analysis in Cancer Diagnosis”
Dr. Madeline O’Donoghue,  "Demonstrating the Utility of the Protein Thermal Shift Assay Using Applied Biosystems Real Time PCR Systems"  Applied Biosystems

CNA - Circulating Nucleic Acids
Analysis of circulating RNAs and DNA and microRNAs as diagnostic and prognostic marker, …
Dr. Pamela Pinzani,  “Cell free circulating DNA”
Dr. Alfred Schöller,  "Targeting the human urine RNAome for tumor diagnostics by qPCR"
Dr. Jim Huggett,  "Diagnostic tools for measuring cell free nucleic acids. What can we expect from the next decade?

Single-cell qPCR
Single-cell sampling, pre-amplification techniques, laser microdissection, sub-cellular PCR, micro-manipulation of cell clusters, cellular micro injection, FACS spotting, single cell handling, pre-amplification…
Dr. Ken Livak,   "High Throughput Gene Expression Profiling of Single Cells"  invited by FLUIDIGM
Dr. Anders Stahlberg,  “Single-cell gene expression profiling”
Dr. Petra Hartmann,  "Analysis of Single Circulating Tumour Cells isolated from Gastro-Intestinal Cancer Patients"  ADVALYTIX
Dr. Stephanie Carl,  "Detection and characterization of single circulating tumor cells as chance to monitor and individualize chemotherapy"    Molecular Machines & Industries

RNAi - microRNA - siRNA Applications – miRNA normalisation
RNAi mechanism, microRNA extraction, qRT-PCR technologies to detect microRNA, microRNA normalisation strategies, siRNA applications in combination with qRT-PCR, microRNA targets and microRNA precursors, new siRNA manipulation and microRNA technologies, ...
Prof. Jo Vandesompele,  “MicroRNA and mRNA gene expression normalization”
Dr. Mirco Castoldi, "Expression profiling of microRNA by quantitative real time PCR, what is available and where to go from there"
Dr. Michael W. Pfaffl,  “Quantification of microRNAs – MIQE challenges and solutions”
Dr. Ditte Andreasen,  "microRNA profiling on challenging samples using LNA enhanced qPCR"  EXIQON
Dr. David Ruff,  "MicroRNA dynamics in early neuronal differentiation with correlation to protein output and cell biology"   Applied Biosystems
Dr. Martin Kreutz,  "Quantification of miRNA precursors in Mammalian Cells"  QIAGEN

qPCR BioStatistics & BioInformatics
software applications, data mining, calculation of relative expression, primer and probe design on mRNA and microRNA level, real-time PCR efficiency determination, mathematical modelling, multivariate expression profiling, statistics in real-time PCR, data management, multiway expression profiling, multiple regression analysis,  3D data visualization, ...

Dr. Ales Tichopad, “Statistical aspects of quantitative PCR experiment design and qPCR data analysis”
Dr. Jan Hellemans, “Accurate and objective copy number profiling using real-time quantitative PCR” BIOGAZELLE
Prof. Mikael Kubista, “Expression profiling - clusters of possibilities” MULTID
Dr. Jan Ruijter, "Determining PCR efficiency and Cq value after monitoring PCR amplification with hydrolysis probes"
Dipl. Inf.  Sebastian Kaiser  "Existing Methods and New Developments for RT-PCR Analysis in R"
Dr. Stefaan Derveaux, "Pros and cons of single curve efficiency algorithms"

Juridicum lecture hall no. 10 with 350 seats.

Event Location:

Public Transport:
  • Straßenbahn (tram): Line 1, 2, D (Stop: Börse), 37, 38, 40, 41, 42, 43, 44 (Stop: Schottentor)
  • U-Bahn (underground): Line U2 (Stop: Schottentor-Universität)
  • Bus: Line 1A (Stop: Helferstorferstraße), 3A, 40A (Stop: Börse)
  • Detailed information about public transport in Vienna:

Industrial Exhibition

An industrial exhibition will be held during the qPCR Symposium 7 – 9 April 2010 in the Aula of the Juridicum (see photo and plan below).
Address:  Juridicum der Universität Wien, Schottenbastei 10-16, 1010 Vienna, Austria
Google Map of Vienna around the conference venue at the University of Vienna - building "Juridicum"
Google Earth LINK to qPCR 2010 conference venue

qPCR 2010 exhibition in Juridicum Aula
=> click to enlarge

qPCR 2010 exhibition in Juridicum Basement
=> click to enlarge

Booth at the industrial exhibition

We have 20 booths in different prize categories available from 7 - 9th April. Prize depends on size and location (see map above). They are located in the Aula of the Juridicum above the lecture hall with can fit 350 people. Built up for the symposium is from Tuesday 6th April noon until 6 p.m. (12:00 to 18:00).
  •  Green booth  (app. 6.5 m long and 3.5 m wide, app. 21 m2,  no. 1, 9 and 24)
  •  Blue booth  (app. 4.2 m long and 3.5 m wide, app. 15 m2,  no. 2 to 8,  21, 22, 26 and 27)
  •  Yellow booth  (app. 4.2 m long and 2.4 m wide, app. 10 m2,  no. 10 to 15 , 23 and 25)
  •  Orange booth  (app. 3.0 m long and 2.0 m wide, app. 6 m2,  no. 16 to 20)

Participating Companies & Sponsors

   Aula - booth no. 1- 19
booth no.

booth no.

booth no.

Roche Diagnostics - LEAD SPONSOR

Bio-Rad - GOLD Sponsor

Lonza - GOLD Sponsor

Integrated DNA Technologies - GOLD Sponsor

Qiagen - GOLD Sponsor


applied biosystems - LEAD Sponsor

TIB Molbiol


Invitrogen - SILVER Sponsor


Fluidigm - GOLD Sponsor


Lab on Net

Biosearch Technologies

Eurofins - GOLD Sponsor

Premier Biosoft - SILVER Sponsor

7 Eurogentec

4titude - Bioline

Integromics - SILVER Sponsor

   Basement - booth no. 20 - 31

20 Biogazelle


Advalytix - GOLD Sponsor

LTF Labortechnik -  Helixis

IT-IS Life Science Ltd  -  GOLD Sponsor


Agilent Technologies - GOLD Sponsor


AlphaMetrix Biotech - BioTrove

25 Molecular Machines & Industries - GOLD Sponsor



Thermo Scientific - GOLD Sponsor



Solis BioDyne

Supporting Journals, Publishers & Media Partners

The talks by the plenary lecturer, selected invited academic and industrial speakers will be published as full papers in METHODS special qPCR issue Vol 50(4) April 2010  with the title “The ongoing evolution of qPCR”  At the meeting all participants will get  a free hard cover of the issue => please view the TOC & HTML or PDF versions

Methods focuses on rapidly developing techniques in the experimental biological and medical sciences. Each topical issue, organized by an editor who is an expert in the area covered, consists of invited articles. Issues are devoted to specific technical approaches with emphasis on clear detailed descriptions of protocols that allow them to be reproduced easily. The background information provided enables researchers to understand the principles underlying the methods; other helpful sections include comparisons of alternative methods giving the advantages and disadvantages of particular methods, guidance on avoiding potential pitfalls, and suggestions for troubleshooting.


Symposium Fees

The registration fees include:
  • Printed proceedings   showing all abstracts of the scientific contributions;
  • Online access to full presentations:   with permission from the authors all presentation slides will be available online as PDF on the symposium homepage, in a password protected area.
  • Full catering service    including all kind of drinks, coffee bar, various cold snacks, lunch sandwich meals and two evening events:  a Wine Reception on Wednesday April 7, and a downtown evening Conference Dinner on Thursday April 8, in the Vienna city center with a very delicious international buffet, alcoholic and non-alcoholic drinks =>
Our online registration and abstract submission tool - CONFTOOL - is available now!


qPCR 2010 Symposium ( 3 days )
Early registration fees
until 31st January 2010

Late registration fees
from 1st February 2010
students *

230 Euro
280 Euro
academic attendants

330 Euro
380 Euro
industrial attendants

430 Euro
480 Euro
VAT regulations for events in Austria:
  • Reverse Charge Taxing System - prices exempt VAT for companies domiciled in EU, CH, USA.  For EU-companies and EU-institutions please deliver your tax ID number.
  • In all other cases (other countries or private persons) prices are additionally 20% Austrian VAT, according to the Austrian § 19 Abs 1 UStG
  • * The students should present a valid student passport at the registration.
If you have further questions, we are pleased to help you =>

Hope to meet you in April in Vienna!

Michael W. Pfaffl


Shipment of bag items:   March 20th 2010

Lise-Meitner-Strasse 30
85354 Freising

Shipment of booth equipment:  
April 6th from 12:00 a.m. to 6.00 p.m. and at April 7th from 8.00 a.m. to 10.00 a.m.   Pick up: April 9th from 3.30 to 6.00 p.m.   Other shippment dates are not possible!
Delivery address:    Juridicum der Universität Wien, Schottenbastei 10-16
at the corner to Heßstrasse, 1010 Vienna, Austria

Hotels next to the Juridicum:
Download hotel contingent list  => Please do your own hotel registrations!

Public transport Vienna:
You will find detailed information about public transport and tickets on:

Every participant of the symposium should register her/himself in our booking system =>
Also exhibitors and invited speaker have to register, using a special registration code which is send via mail. Using this code no fees will be charged for booth members and invited speakers.

Social Program:
Wednesday, April 7th - Wine reception at the Juridicum
Thursday, April 8th - Conference Dinner downtown in Vienna =>

Speaker - Information:
coming soon

Symposium fees
Student prices can only be booked with a valid matriculation number by studens and PhD students. We do not have traveling or accomodation grants for the event.

Wireless LAN will be provided. Login details at the event.

© 2009-2010
BioEPS GmbH,  Lise-Meitner-Strasse 30,  8 5354 Freising    Vertretungsberechtiger Geschäftsführer: Dr. Martina Reiter
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