• Online Agenda  =>  http://agenda.qpcr2010-vienna.net
• The talks by the plenary lecturer, selected invited academic and industrial speakers will be published in METHODS special qPCR Vol 50 issue 4  (April 2010)  with the title “The ongoing evolution of qPCR”.   At the meeting all participants will get  a free hard cover of the issue => please view the TOC  and  HTML or PDF versions
• Download info flyer - Vienna-qPCR-2010-final-announcement.pdf
  
• Final Call for Abstracts  =>  call-for-abstracts-qPCR-2010.pdf  -  This time we can only accept abstracts for talks!
  
• Conference Dinner downtown in Vienna at Thursday April 8th => http://www.zwoelf-apostelkeller.at/index-en.htm
• 39 companies participate at the industrial exhibition
• Download hotel contingent list  => Please do your own hotel registrations!
• Online registration and abstract submission tool - CONFTOOL  -   Please register here  =>  http://registration.qpcr2010-vienna.net
• Download the official Press Release => press-release-qPCR-2010.pdf

qPCR Symposium topic  - “The ongoing evolution of qPCR”

The symposium focus will be on 40 lectures presented by international recognised experts in their application fields. The emphasis will be on unbiased, didactic information exchange. One third of the talks will be presented by invited speakers, one third of the speakers will be selected from the submitted abstracts and one third will be qPCR company representatives with their newest qPCR technologies.

The focus of the qPCR 2010 Event will be  “The ongoing evolution of qPCR”  representing all new and emerging techniques, applications and data analysis methods:

Symposium sessions & preliminary titles

MIQE and QM strategies in qPCR
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Following these guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of qPCR results. QM strategies in real-time PCR to guarantee better and more valid results.  
Prof. Stephen Bustin,  “The MIQE Guidelines:  Minimum Information for Publication of Quantitative Real-Time PCR Experiments”
Prof. Christine Mannhalter,  "Standardization efforts of qPCR: Example BCR-ABL Translocation"
Dr. Roderic Fürst,   "Optical artefacts: Cause, Consequence, Correction"  IT-IS Lifescience
Dr. Mark Behlke,   "Hydrolysis probes with improved quenching and performance"  INTEGRATED DNA TECHNOLOGIES
Dr. Alexander Rácz,  "Advanced qPCR - one step ahead with next generation dyes."   Eurofins MWG Operon
Dr. Francisco Bizouarn,   "A Practical Approach to Assay Design for qPCR - Overcoming Difficult Assays, Designs and Optimization while Conforming to the MIQE Guidelines"   Bio-Rad
Dr. Ian Kavanagh,   "Detection of Related AKT Family Members using Solaris qPCR Gene Expression Assays"   Thermo Scientific

High throughput quantitative PCR – digital PCR
384 well applications, new high throughput platforms, droplet PCR, qPCR robotics, digital PCR, gene expression real-time RT-PCR arrays (mRNA and microRNA), quantitative multiplexing, …
Prof. Mikael Kubista, “Digital PCR and intra-cellular expression profiling” TATAA BIOCENTER
Dr. Philip Day, “High throughput droplet PCR”
Dr. Gudrun Tellmann, "High-Throughput Gene Expression Analysis Using the LightCycler Platform" ROCHE
Dr. Leila Smith, "Fluidigm’s BioMark System for Gene Expression, Genotyping, Digital PCR and other sample preparation applications using nanoliter reaction volumes" FLUIDIGM
Dr. Marjaana Pussila, "Western-type diet affects the expression of genes known to be involved in human colorectal carcinogenesis in histologically normal mucosa of ApcMin/+ mice" invited by LONZA
Dr. Simone Kreth, "Prognostic Impact of Gene Expression Analyses in Human Glioblastoma" invited by ROCHE
Dr. Raza Ahmed, "Rapid Quantification of DNA libraries for Next Generation Sequencing"  Agilent Technologies
Dr. Ian Kavanagh, "Detection of Related AKT Family Members using Solaris qPCR Gene Expression Assays"  Thermo Scientific

HRM – High Resolution Melting - Epigenetics
SNP analysis, HRM = high resolution melt applications, Epigenetics, methylation markers, HRM platform comparison, etc …
Prof. Carl Wittwer, "High Resolution Melting Analysis"
Prof. Claudio Orlando, “High Resolution Melting Analysis in Cancer Diagnosis”
Dr. Madeline O’Donoghue,  "Demonstrating the Utility of the Protein Thermal Shift Assay Using Applied Biosystems Real Time PCR Systems"  Applied Biosystems

CNA - Circulating Nucleic Acids
Analysis of circulating RNAs and DNA and microRNAs as diagnostic and prognostic marker, …
Dr. Pamela Pinzani,  “Cell free circulating DNA”
Dr. Alfred Schöller,  "Targeting the human urine RNAome for tumor diagnostics by qPCR"
Dr. Jim Huggett,  "Diagnostic tools for measuring cell free nucleic acids. What can we expect from the next decade?”

Single-cell qPCR
Single-cell sampling, pre-amplification techniques, laser microdissection, sub-cellular PCR, micro-manipulation of cell clusters, cellular micro injection, FACS spotting, single cell handling, pre-amplification…
Dr. Ken Livak,   "High Throughput Gene Expression Profiling of Single Cells"  invited by FLUIDIGM
Dr. Anders Stahlberg,  “Single-cell gene expression profiling”
Dr. Petra Hartmann,  "Analysis of Single Circulating Tumour Cells isolated from Gastro-Intestinal Cancer Patients"  ADVALYTIX
Dr. Stephanie Carl,  "Detection and characterization of single circulating tumor cells as chance to monitor and individualize chemotherapy"    Molecular Machines & Industries

RNAi - microRNA - siRNA Applications – miRNA normalisation
RNAi mechanism, microRNA extraction, qRT-PCR technologies to detect microRNA, microRNA normalisation strategies, siRNA applications in combination with qRT-PCR, microRNA targets and microRNA precursors, new siRNA manipulation and microRNA technologies, ...
Prof. Jo Vandesompele,  “MicroRNA and mRNA gene expression normalization”
Dr. Mirco Castoldi, "Expression profiling of microRNA by quantitative real time PCR, what is available and where to go from there"
Dr. Michael W. Pfaffl,  “Quantification of microRNAs – MIQE challenges and solutions”
Dr. Ditte Andreasen,  "microRNA profiling on challenging samples using LNA enhanced qPCR"  EXIQON
Dr. David Ruff,  "MicroRNA dynamics in early neuronal differentiation with correlation to protein output and cell biology"   Applied Biosystems
Dr. Martin Kreutz,  "Quantification of miRNA precursors in Mammalian Cells"  QIAGEN

qPCR BioStatistics & BioInformatics
software applications, data mining, calculation of relative expression, primer and probe design on mRNA and microRNA level, real-time PCR efficiency determination, mathematical modelling, multivariate expression profiling, statistics in real-time PCR, data management, multiway expression profiling, multiple regression analysis,  3D data visualization, ...
Dr. Ales Tichopad, “Statistical aspects of quantitative PCR experiment design and qPCR data analysis”
Dr. Jan Hellemans, “Accurate and objective copy number profiling using real-time quantitative PCR” BIOGAZELLE
Prof. Mikael Kubista, “Expression profiling - clusters of possibilities” MULTID
Dr. Jan Ruijter, "Determining PCR efficiency and Cq value after monitoring PCR amplification with hydrolysis probes"
Dipl. Inf.  Sebastian Kaiser  "Existing Methods and New Developments for RT-PCR Analysis in R"
Dr. Stefaan Derveaux, "Pros and cons of single curve efficiency algorithms"

Juridicum lecture hall no. 10 with 350 seats.

• Juridicum der Universität Wien, Schottenbastei 10-16, 1010 Vienna, Austria
• Google Map of Vienna around the conference venue at the University of Vienna - building "Juridicum"
• Google Earth LINK to qPCR 2010 conference venue

• Straßenbahn (tram): Line 1, 2, D (Stop: Börse), 37, 38, 40, 41, 42, 43, 44 (Stop: Schottentor)
• U-Bahn (underground): Line U2 (Stop: Schottentor-Universität)
• Bus: Line 1A (Stop: Helferstorferstraße), 3A, 40A (Stop: Börse)
• Detailed information about public transport in Vienna:  http://www.wienerlinien.at/wl/ep/home.do?tabId=0

Industrial Exhibition

=> click to enlarge

=> click to enlarge

Booth at the industrial exhibition

We have 20 booths in different prize categories available from 7 - 9th April. Prize depends on size and location (see map above). They are located in the Aula of the Juridicum above the lecture hall with can fit 350 people. Built up for the symposium is from Tuesday 6th April noon until 6 p.m. (12:00 to 18:00).

•  Green booth  (app. 6.5 m long and 3.5 m wide, app. 21 m2,  no. 1, 9 and 24)
•  Blue booth  (app. 4.2 m long and 3.5 m wide, app. 15 m2,  no. 2 to 8,  21, 22, 26 and 27)
•  Yellow booth  (app. 4.2 m long and 2.4 m wide, app. 10 m2,  no. 10 to 15 , 23 and 25)
•  Orange booth  (app. 3.0 m long and 2.0 m wide, app. 6 m2,  no. 16 to 20)

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Participating Companies & Sponsors

Aula - booth no. 1- 19
booth no.		booth no.		booth no.
1	Roche Diagnostics - LEAD SPONSOR	8	Bio-Rad - GOLD Sponsor	15	Lonza - GOLD Sponsor
2	Integrated DNA Technologies - GOLD Sponsor	9	Qiagen - GOLD Sponsor	16	BioEPS
3	applied biosystems - LEAD Sponsor	10	TIB Molbiol	17	TATAA
4	Invitrogen - SILVER Sponsor	11	IMGM	18	MultiD
5	Fluidigm - GOLD Sponsor	12	BIOKE	19	Lab on Net
6	Biosearch Technologies	13	Eurofins - GOLD Sponsor		Premier Biosoft - SILVER Sponsor
7	Eurogentec	14	4titude - Bioline		Integromics - SILVER Sponsor
Basement - booth no. 20 - 31
20	Biogazelle	24	Advalytix - GOLD Sponsor LTF Labortechnik -  Helixis	27	IT-IS Life Science Ltd  -  GOLD Sponsor
21	Agilent Technologies - GOLD Sponsor			28	EXIQON - GOLD Sponsor
22	AlphaMetrix Biotech - BioTrove	25	Molecular Machines & Industries - GOLD Sponsor	29	Biolegio
23	Finnzymes	26	Thermo Scientific - GOLD Sponsor	30	Eppendorf
				31	Solis BioDyne

Supporting Journals, Publishers & Media Partners

The talks by the plenary lecturer, selected invited academic and industrial speakers will be published as full papers in METHODS special qPCR issue Vol 50(4) April 2010  with the title “The ongoing evolution of qPCR”  At the meeting all participants will get  a free hard cover of the issue => please view the TOC & HTML or PDF versions Methods focuses on rapidly developing techniques in the experimental biological and medical sciences. Each topical issue, organized by an editor who is an expert in the area covered, consists of invited articles. Issues are devoted to specific technical approaches with emphasis on clear detailed descriptions of protocols that allow them to be reproduced easily. The background information provided enables researchers to understand the principles underlying the methods; other helpful sections include comparisons of alternative methods giving the advantages and disadvantages of particular methods, guidance on avoiding potential pitfalls, and suggestions for troubleshooting.

• Printed proceedings   showing all abstracts of the scientific contributions;
• Online access to full presentations:   with permission from the authors all presentation slides will be available online as PDF on the symposium homepage, in a password protected area.
• Full catering service    including all kind of drinks, coffee bar, various cold snacks, lunch sandwich meals and two evening events:  a Wine Reception on Wednesday April 7, and a downtown evening Conference Dinner on Thursday April 8, in the Vienna city center with a very delicious international buffet, alcoholic and non-alcoholic drinks => http://www.zwoelf-apostelkeller.at/index-en.htm