qPCR 2009 Symposium Talks

keynote speaker: preliminary title

The Pioneer in PCR
Kary B. Mullis

1993 Nobel Prize in Chemistry

In 1993, the Royal Swedish Academy of Sciences awarded the Nobel Prize in Chemistry for work on DNA-based chemistry methods, with one-half of the prize going to Kary Mullis for his invention of the polymerase chain reaction (PCR) method. His work, the academy said, had "hastened the rapid development of genetic engineering" and "greatly stimulated biochemical research and opened the way for new applications in medicine and biology."
The academy added: "The applications of Mullis' PCR method are already many. It is for example possible using simple equipment to multiply a given DNA segment from a complicated genetic material millions of times in a few hours, which is of very great significance for biochemical and genetic research. The method offers new possibilities particularly in medical diagnostics, and is used, for example, for discovering HIV virus or faulty genes in hereditary diseases."

25th Anniversary of PCR

Here's the academy's press release announcing the award, with more information about PCR => http://www.karymullis.com/
lectures => http://www.karymullis.com/new/texte/lectures.php

Molecular Diagnostics

qPCR NOS Session
Normalization & Optimization & Standardization
Stephen A. Bustin
Professor of Molecular Science, Institute of Cell and Molecular Science, Queen Mary's School of Medicine and Dentistry, University of London, UK

Stephen Bustin obtained his PhD from Trinity College, University of Dublin in molecular genetics in 1983. Since 1989 he has worked at the Royal London Hospital, aiming to apply his research in a more direct, practical setting. Following promotion to Senior Lecturer (1995) and Reader in Molecular Medicine (2002) he was awarded a personal chair by the University of London in 2004. He was appointed as a visiting Professor of Molecular Biology by the University of Middlesex in 2006.
His main area of research is into bowel-associated pathologies, especially colorectal cancer and, more recently, Clostridium difficile-associated disease. He has a special interest in molecular technologies and his laboratory operates at the forefront of technological development in nucleic acid quantification, where he is an internationally acknowledged leader. He has published numerous peer-reviewed papers and reviews and is the editor of the “A-Z of quantitative PCR”, the leading textbook for this technology. He is on the editorial boards of several journals and has given numerous presentations at scientific conferences around the world. He has organized and co-organised many qPCR meetings in the UK, Europe and the US.

A new qPCR assay for the detection of Clostridium difficile


MIQE- guidelines for publication of qPCR data

High Throughput

Ken Livak
Senior Scientific Fellow, Fluidigm Corporation,San Francisco, CA, US

Dr. Livak has recently joined Fluidigm Corporation as Senior Scientific Fellow and is charged with pioneering novel assays on Fluidigm’s microfluidic platform.  During his 13 years at Applied Biosystems, he concentrated on improving the robustness and versatility of using fluorogenic (TaqMan) probes in the 5’ nuclease PCR assay.  Accomplishments include the discovery of a general method for probe design, application of fluorogenic probes to allelic discrimination, and elucidation of how to use real-time PCR data for quantification of nucleic acids.  As Vice President, Science, he reviewed and coordinated scientific efforts across all the instrument platforms developed by Applied Biosystems in order to foster technology innovation in the broad area of life science discovery.

Moving from qPCR Assays to qPCR Arrays.

invited academic speaker:
preliminary title

Molecular Diagnostics

RNAi - microRNA - siRNA Applications
Jo Vandesompele
Center for Medical Genetics Ghent, Ghent University Hospital
, Ghent, Belgium
Multi-gene signature for cancer patient survival.

Normalization of miRNA data.

Molecular Diagnostics
Andreas Nitsche
Robert Koch Institut ZBS 1, Berlin, Germany

Real-time PCR based Diagnostic of highly pathogenic Viruses.
Single-cell qPCR Anders Stahlberg
Stem Cell Center
Lund University, Lund, Sweden

Technical aspects of mRNA quantification in single cells using RT-qPCR.
RNAi - microRNA - siRNA Applications Greg Shipley
Director, Quantitative Genomics Core, Laboratory, The University of Texas Health Science Centre- Houston, TX, US

A Multi-assay Approach to the Study of Cellular Toxicity.
Bioinformatics Mikael Kubista
Professor of Biotechnology, TATAA Biocenter Sweden, MultiD Analyses AB, Göteborg, Sweden

Gene Expression Profiling with qPCR.
Copy Number Variation
Jan Helemanns
Center for Medical Genetics Ghent, Ghent University Hospital
, Ghent, Belgium
Biogazelle NV, Kruishoutemstraat 57, Zulte, Belgium

Accurate and objective copy number profiling.
Molecular Diagnostics Dimitrios Frangoulidis
Institut für Mikrobiologie der Bundeswehr, Munich, Germany

Real-time PCR of bioterrorism agents.
Molecular Diagnostics Olfert Landt
TIB MOLBIOL Syntheselabor GmbH, Eresburgstrasse, Berlin, Germany

Probe Based Detection of Genetic Variations - Screening and in-vitro Diagnostics.
Pre-PCR optimisation Michael W. Pfaffl
Reader in Physiology, Center of Life Science, Weihenstephan, Technical University of Munich, Freising, Germany

mRNA & microRNA integrity - the key to success.
Single-cell qPCR Birgit Liss
Institut für Allgemeine Physiologie, Universität Ulm, Germany

qPCR of indicidual dopamine neurons form mouse models and human post mortem brain sections.
Molecular Diagnostics Jim Huggett
Centre for Infectious Diseases & International Health, University College London, UK

The use of nucleic acid amplification tests for research and diagnosis in the developing world.

RNAi - microRNA - siRNA Applications Christian Thirion
Managing Director, SIRION GmbH, Planegg/Martinsried, Germany

An inflammatory microRNA signature in muscle cells - a comparative study of cellular models and technological platforms.

Molecular Diagnostics Udo Reischl
Institut für Medizinische Mikrobiologie und Hygiene - Universität Regensburg, Germany

Current applications of real-time PCR technology in diagnostic bacteriology.
Bioinformatics Ales Tichopad
Labonnet LTD / Technical University Munich, Physiology Weihenstephan

Adequate experiment design as the first important step in obtaining valid biological inference with qPCR technique.


Filip Pattyn
Center for Medical Genetics Ghent, Ghent University Hospital
, Ghent, Belgium

New RTPrimerDB.
Molecular Diagnostics Pamela Pinzani
Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, Florence, Italy

Circulating nucleic acids in melanoma diagnosis.
Bioinformatics Anders Bergkvist
Senior Scientist, Head of Business Development MultiD Analyses AB, Sweden

Data analysis for gene quantification and expression profiling using GenEx.

RNAi - microRNA - siRNA Applications
Mirco Castoldi
EMBL Heidelberg, Heidelberg, Germany

miQPCR - a novel approach for expression profiling of mature microRNAs.
Molecular Diagnostics Hans Nitschko
Max von Pettenkofer-Institut, Ludwig-Maximilians-University Munich, Department of Virology, Germany

Multiplex-PCR in clinical virology - benefits and limitations.
Molecular Diagnostics
in agricultural and veterinary Science
Irmgard Riedmaier
Physiology Weihenstephan, Center of Life Science, Technical University of Munich, Freising, Germany

The use of transcriptomics for biomarker development to trace anabolic hormone functions.
Molecular Diagnostics
in agricultural and veterinary Science

Huber Ingrid
Bavarian Health and Food Safety Authority, Germany

Rapid detection and differentiation of Campylobacter jejuni, C. coli and C. lari in food samples using a quadruplex real-time PCR assay.

Bioinformatics Jochen Blom
Center for Biotechnology, Bielefeld University, Germany

CAmpER - An open analysis framework for real-time PCR data using single sample amplification efficiency calculation.

Molecular Diagnostics Kim De Leeneer
Center for Medical Genetics Ghent (CMGG),  Ghent University Hospital, Medical Research Building (MRB), De Pintelaan 185, Ghent, Belgium

invited by GOLD SPONSOR Bio-Rad Laboratories, Hercules, US
Applications of HRM Curve Analysis: Strengths and Pitfalls.

industrial R&D speaker:
preliminary title


High Throughput Thomas Froehlich
Director R&D, Roche Applied Science, Mannheim, Germany

LightCycler 1536 System - Introducing the next Level of High-Throughput PCR.
qPCR NOS Session Ralf Mauritz
Director R&D, Roche Applied Science, Mannheim, Germany

RealTime ready – Functionally Tested Assays for Gene Expression Analysis on the LightCycler Platform.

High Throughput Ken Livak
Senior Scientific Fellow, Fluidigm Corporation,San Francisco, CA, US

Moving from qPCR Assays to qPCR Arrays.
RNAi - microRNA - siRNA Applications Jason Halsey
VP of Molecular Biology Systems R&D,
Applied Biosystems, R&D, US

Discovery and Validation of Novel Human MicroRNA Genes by SOLiD and TaqMan.
Molecular Diagnostics Debra Nickson
Regional Business Manager, Gene Expression Profiling, Invitrogen, United Kingdom

Gene Scanning by High-Resolution Melt (HRM) using EXPRESS SYBR GreenER qPCR SuperMix.

qPCR NOS Session Steffen Müller
Agilent Technologies Inc, Stevens Creek Blvd, Santa Clara , CA, US

Importance of experimental design and sample QC for robust and meaningful QPCR results.

RNAi - microRNA - siRNA Applications Martin Kreutz
Scientist R&D – miRNA Research, QIAGEN GmbH, Hilden, Germany

Quantification and Functional Analysis of miRNA in Mammalian Cells.
High Throughput Ben Sowers
Biosearch Technologies, Novato, CA,

Oligo Design Across the Mouse Genome.
qPCR NOS Session Ian Kavanagh
Abgene, Thermo Fisher Scientific, UK

Increasing qPCR throughput: simple steps to speed up results whilst minimising variance.

Molecular Diagnostics Jim Thorn
Product Manager Genetic Analysis, Beckman Coulter GmbH - Diagnostics, Europark Fichtenhain, Krefeld,  Germany

A Novel Multiplex, Quantitative Gene Expression Approach for Cancer Biomarker Research.
RNAi - microRNA - siRNA Applications Ditte Andreasen
Exiqon, R&D, Vedbaek, Denmark

Highly sensitive and specific LNA enhanced real-time PCR for microRNA expression analysis.


25th Anniversary of PCR Nobel Prize Laureate Kary Mullis
sponsored by

Biosearch Technologies, Novato, CA,

25th Anniversary of PCR.
qPCR NOS Session
Daniel J. Shaffer
Vice President and Founder, Bar Harbor BioTechnology, Inc., USA

Highly Accurate Quantitative Gene Expression Analysis without Use of pre-defined Normalizer Genes using Pattern Recognition Analysis.

Diagnostics & Molecular Markers
Rob Powell
PrimerDesign, Millbrook Technology Campus, Southampton Hants, UK

High resolution melt analysis for low resolution machines.

High throughput qPCR Natasha Paul
TriLink BioTechnologies, San Diego, CA, USA

The Next Generation in Hot Start PCR - CleanAmp Primers and dNTPs.

Single-cell qPCR Jim White
Business Development, BioTrove Inc., USA
Alpha Metrix Biotech, Rödermark, Germany

Measurement of Gene Expression by Massively Parallel Nanoliter real-time PCR.
RNAi - microRNA - siRNA Applications Carola Wagner
IMGM Laboratories GmbH, Martinsried, Germany
microRNAs - developing new tools for diagnostics - Join forces with IMGM Laboratories to make your miRNA project a success.

All scientific contributions will be published in the qPCR 2009 Symposium Proceedings
Any changes ???   =>   please contact the scientific organizer Michael W. Pfaffl via    qPCR2009@wzw.tum.de