Call for "Late Breaking Poster" Submissions @ GQ2019
deadline is extended to 1st March 2019

9th Gene Quantification Event
qPCR dPCR & NGS 2019

Symposium  &  Industrial Exhibition  &  Application Workshops
18 - 22 March 2019, in Freising-Weihenstephan,
School of Life Sciences, Technical University of Munich, Weihenstephan, Germany

focus topics:
Next Generation Biomarkers -- Liquid Biopsy, Multi-Omics, MicroGenomics

Registration & submission platform =>

Symposium Agenda is Online =>

Agenda PDF version =>

Talk & Poster Sessions to be presented at the event:

=> All Confirmed Symposium Speakers

Main topic:   Next-Generation Biomarkers
The session is about the discovery, detection and validation of molecular biomarkers:  diagnostic, prognostic, clinical biomarkers on DNA, RNA, microRNA, lncRNA, small RNA, metabolome and proteome level (e.g. disease markers, cancer or stem-cells markers, tissue specific markers, differentiation markers, methylation markers). New assay systems from high-throughput genomics and proteomics will be presented.

Main topic:   Liquid Biopsy & Circulating Nucleic Acids
The capture of whole circulating tumor cells (CTCs) was the initial focus of liquid biopsy. Today, it also includes the collection and analysis of cell-free nucleic acids to provide useful information on the physiological status. The session includes any kind of circulating nucleic acids families (DNA, RNA, small RNAs, microRNAs, piRNAs, & lncRNAs). Extracellular Vesicles & exosomes have their own session!

Main topic:   Multi-Omics & Integrative Big Data Analysis
This session focuses on the application of highly sophisticated methods, applications and algorithms to discover, detect, and validate molecular biomarker signatures. A special focus is put on the integrative analysis of multi-omics or multi-level biomarkers, e.g. microRNA / mRNA / lncRNAs, and the complex analysis of genomic, proteomic, metabolomic & phenotypic markers.

Main topic:   MicroGenomics & Single-Cells Diagnostics
The focus of this session is on micro-genomics, the application of molecular methods to detect biomarkers in a minimal amount of tissue or any other biological matrix. New applications in single-cell biology will be presented: isolation, separation, characterization technologies, laser-microdissection, capturing of circulating tumor cells (CTC), pre-amplification techniques, sub-cellular PCR, micro-manipulation of cell clusters, cellular micro injection, single-cell handling, FACS sorting and spotting.

ISEV Satellite Session – EV & Exosome Diagnostic Biomarkers         
The broader scope of this session covers the isolation, purification, quantification and characterization of extracellular vesicles (EVs) such as exosomes and microvesicles as well as the analysis of corresponding biomarkers (intra-vesicular and membrane-associated The focus will be on EV transcriptomics & genomics, and the connected integrative bioinformatics, e.g. integrative data analysis and isomiR analysis for exosomal biomarker discovery in any clinical or Life Science context.
Digital PCR
Digital PCR (dPCR) can be used to directly quantify and clonally amplify nucleic acids including DNA, cDNA, mRNA, microRNA or any other small RNA species. It allows a more reliable and highly sensitive measurement of nucleic acid quantities as well as applications in copy number variants (CNV) and analysis of point mutations in molecular diagnostics.

Non-coding RNAs -- microRNA, isomiRs, small RNAs, long non-coding RNAs
This session is dedicated to the family of non-coding RNA and its RNAi mechanism and applications. A new focus will be on the identification and data analysis & modelling of new isomiR biomarkers and long non-coding RNAs. RT-qPCR or small RNA-Seq technologies to quantify small & long non-coding RNAs and other classes of small RNAs, such as piRNAs or tRNAs. siRNA knockdown applications, microRNA targets and microRNA precursors, new siRNA manipulation, etc. will be presented.

Next-Generation Sequencing (NGS)
NGS applications offer new holistic analysis of any kind of nucleic acid to investigate the Genome, Exome, Epigenome, Transcriptome and Splicome (total RNA, microRNA, small RNA, long ncRNA) as well as CHIP-purified nucleic acids, etc. Various NGS topics will be presented: “NGS overview talks - information technology in the era of NGS & diagnostic applications”;   “Pre NGS - sample preparation & setup & library generation”;   “NGS – new sequencing technologies e.g. in-situ, single molecule and pore sequencing”;   NGS – data analysis: data management, mapping, alignment algorithms, data de novo assembly.

qPCR & NGS data analysis
The data analysis in RT-qPCR expression profiling and NGS experiments is still challenging and time consuming. It is well known that a lot of error and variation is introduced by incorrect experimental study design, poor sample and group normalisation or differential gene expression analysis. This session is focused on the application of useful new algorithms & data analysis methods to get better, more reliable and MIQE-compliant results, e.g. R-based freeware, and software applications for data mining and comparison, calculation of relative expression. One focus of this session will be on mathematical modelling, multivariate expression profiling, statistics in NGS and qPCR, multiple regression analysis, 3D data visualization, and more.

Advanced Molecular Diagnostics in Life Science
This session is dedicated to molecular diagnostics in Life Science, with focus on Agriculture, Veterinary Medicine, Food and Environmental Science, which is the major research focus at the TUM School of Life Science Campus Weihenstephan. All applications of highly sophisticated quantification methods, field application or algorithms to research the wide field of agro-veterinarian, food or life science are very welcome.

MIQE & Quality Management & Standardisation Strategies in Molecular Diagnostics
This session focuses on standardization strategies and quality management in research & molecular diagnostics. The goal is to guarantee better and more valid gene quantification results. Of special interest in the context of qPCR or dPCR are the MIQE guidelines (Minimum Information for publication of Quantitative real-time PCR Experiments). Following these guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of qPCR and dPCR results.

Technical Lunch-Time Seminars

For commercial and industry talks (20-25 min talk and 5-10 min discussion).

=> All Confirmed Symposium Speakers

Important dates:

Abstract Submission Deadline:
"Late Breaking Poster" abstract submission deadline is 1st March 2019

Please register using the Internet based ConfTool registration & submission platform =>

  1. Register as ConfTool User  =>  NEW USER  =>  Create new account
  2. Register as symposium PARTICIPANT  =>  for the symposium and/or workshops
  3. Submit Contribution  =>  submit your TALK and/or POSTER  abstract(s)
  4. Please indicate in which Session you want to present either a POSTER or give an TALK Presentation via MS PowerPoint
  5. The abstract should be a one-page document based on unpublished material and written in good standard English.
  6. The text should clearly state objectives, methodology, results, and conclusion. Simple tables and graphs are allowed.
  7. All scientific contributions (talks and poster abstracts) will be published in the event proceedings.
  8. Please note -- All talks will be recorded!
    In summer 2019 the recorded talks will be published on
    Until autumn 2019 the recorded tallks are password protected and only accessible for Symposium and Workshop participants.
  • The official conference and workshop language is ENGLISH
  • No simultaneous translation will be provided.
  • All oral presentations, posters and abstracts must be presented in English
Poster Layout:
  • The official conference and workshop language is ENGLISH
  • All oral presentations, posters and abstracts must be presented in English
  • Until autumn 2019 the PDF download of the poster contributions are password protected and only accessible for Symposium and Workshop participants.
  • Poster will be DIN A0   (portrait format  =>  120 cm/48 inch high x 90 cm/35 inch wide)
  • Presentation of every communication at the Conference is subjected to formal registration of at least one co-author.
Symposium Talks:
(each talk 30 min)
  • Monday             18th March 2019        10:00 a.m.  to  6:00 p.m.
  • Tuesday            19th March 2019         8:30 a.m.  to   6:30 p.m.
  • Wednesday        20th March 2019         8:30 a.m.  to   5:00 p.m.

Please direct your enquiry to our scientific organisation team, headed by Michael W. Pfaffl
©  2018-2019