Links to the WWW            qPCR Newsgroups

Newsgroups,  e-Groups,  Mailing Alert,  Discussion Forums,  Books,  etc.....

please follow this link to our new  =>  qPCR INFO PORTAL

Can't find the right information you want in my sites?  Try a Google search!

    Web based Dictionaries, Glossaries & Protocols:

The Web Guide of Polymerase Chain Reaction Technique.
for further information please contact

CYCLERtest is an independent company dedicated to perform and to   develop the most sophisticated and accurate multichannel
 temperature validation systems, tests and techniques available on  the market at this point in time, all of which must be compliant with and related to reliable industry standards.

PCR  and  RT-PCR

PCR  and  RPA   &  others  movies under

by Dolan DNA Learning Center,  Cold Spring Habor Laboratory

The Polymerase Chain Reaction ( PCR ) Jump Station

The ultimate Web page for information and links on all aspects of the Polymerase Chain Reaction
The polymerase chain reaction (PCR) is used in a wide variety of fields including: molecular biology, environmental science, forensic science, medical science, biotechnology, microbiology,  the food industry, diagnostic science, epidemiology, genetics, gene cloning, and many more. This site provides scientists with a comprehensive directory for the polymerase chain reaction (PCR) and related techniques.

The center for qPCR in Europe

The Lundberg laboratory was among the pioneers in quantitative real-time PCR and contributed by inventing the light-up probes. We now strengthen our capability to handle external relations and take on commercial projects within the field of Q-PCR.

Amongst TATAA Biocenter activities:

  • Commissioned research and development projects
  • Test & Training: Try Q-PCR, compare platforms and chemistry
  • hands-on workshops and seminars, etc
  • Design of Q-PCR assays

PCR and multiplex PCR: guide and troubleshooting

Designed by OCTAVIAN HENEGARIU, Yale University


Quantitative Real-Time Polymerase Chain Reaction for the Core Facility Using TaqMan and the Perkin-Elmer/Applied Biosystems Division 7700 Sequence Detector 

by  Deborah S. Grove 
Nucleic Acid Facility, Life Science Consortium, The Pennsylvania State University, University Park, PA 16802


TechNotes 8 (1):    real-time PCR Goes Prime Time

Real-time PCR assays used for quantitative RT-PCR combine the best
attributes of both relative and competitive (end-point) RT-PCR in that they
are accurate, precise, capable of high throughput, and relatively easy to
perform. To truly appreciate the benefits of this technology, a review of PCR
fundamentals is necessary.


PCR and multiplex PCR: guide and troubleshooting

PCR Applications Manual. Roche Molecular Biochemicals-Research

Alkami Biosystems Primer Design Tips

BioGuide - PCR

PCR-based sequence tagged sites have been used as landmarks for construction of various types of genomic maps. Using NCBI's "electronic PCR" program, these sites can be detected in DNA sequences, potentially allowing their map locations to be determined.

Polymerase Chain Reaction (PCR) Jump Station - An excellent web page for links and information on PCR.

Amplification Assistant PCR Troubleshooting Program.

DNA and PCR methods

  REAL-TIME  PCR by  M.Tevfik Dorak, MD, PhD

Applied Biosystems Sequence Detection Systems  and   ABI User Bulletins

Applied Biosystems Sequence Detection Systems   -  NEWS

ABI-PRISM  7700 FAQs   &    Tutorials    &   Technical Notes   &   Multimedia Presentations

Products for LightCycler

Transcript of a Webcast on Real-Time PCR Applications (Bio.Com)

An excellent review on Quantitative Real-Time PCR   (Bustin SA, 2000)

A review on Product Differentiation by DNA Melting Point Curves (Ririe et al, 1997)

QiaGen Handbooks on SYBR Green Detection Systems and RT-PCR   and SYBR Green Kit

A Biocompare article on real-Time PCR platforms

A Review of Molecular Beacons   (by  Robinson JK et al. )

A technical article on Fluorescence Excitation and Emission on IDT Website

Quick Guide for PCR Vol. I

The COMPLETE Alkami Quick GuideTM for PCR is a 
158 page manual with a file size of 296 KB. It is formated 
to be 5.5" X 8.5" in order to make the Guide a convenient 
size for use at your bench. The complete Guide will print 
out on 79 single-sided, letter sized, 8.5" X 11" sheets 
which you can cut and bind as desired. Click [HERE] to 
download your FREE copy of the Adobe Acrobat Reader.


PCR Primer design
PCR Methods
PCR Polymerases
PCR Variables
PCR Troubleshooting

Special PCR Topics

Comprehensive Index 

PCR Primers
PCR Methods
PCR Product Guides
Alkami PCR Reviews:
Primer Design Software
Alkami PCR Reviews:
PCR Methods
Alkami PCR Reviews:
PCR Products
Online Primer Design
PCR Troubleshooting
FREE Product Offers
Primer Design Tips
PCR Methods
Online BioSuppliers
PCR Discussions
PCR Publications
Alkami PCR Reviews
BioScience Job Links
Alkami Biosystems
Online Journals
Alkami Editorial Staff
PCR Books
Biotech News


Applications of Rapid Cycle real-time PCR

by  Carl Wittwer is Professor of Pathology at the University of Utah Medical School and Director of 
Flow Cytometry and New Technology at Associated Regional and University Pathologists (ARUP), 
and Chief Technology Officer at Idaho Technology.

Applications of Rapid Cycle Real Time PCR

This presentation requires the RealPlayer 8 Basic

Quantification on the LightCycler® Instrument  

Calculation of the efficiency, "fit point method" vs. "2nd derivate maximum method", noise-band, hybridisation probes, etc.
by  Randy Rasmussen Ph.D.  (Idaho Technology, Chief Operating Officer)

Horgan GW   &  Rouault J   (2000) 
Introduction to randomisation tests, Biomathematics and Statistics Scotland,

Explorapedia of Statistical and Mathematical Techniques for use in Research & Technology