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► Invitrogen’s
qPCR product portfolio combines Invitrogen’s superior enzymes and
Molecular Probes™ labeling and detection technology to deliver
sensitive. In addition, Invitrogen is committed to delivering the
latest in advanced qPCR technologies to deliver maximum reliability,
convenience and throughput for your gene expression experiments.
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SYBR® GreenER™
the Next Generation in Real-Time qPCR Detection
The SYBR® GreenER™ qPCR reagent system incorporates the latest
technology to produce the most reliable gene expression data from
real-time qPCR. The SYBR® GreenER™ system is specially formulated
to offer the best sensitivity, specificity, and reproducibility with:
- Novel dsDNA-binding
dye - Exhibits a brighter signal and reduced PCR inhibition
compared to original SYBR® Green dye
- Highly purified
hot-start Taq polymerase - Minimizes introduction of DNA
contamination
- Optimized buffer
system - Improves specificity and low-copy detection
- Uracil-DNA-glycosylase/dUTP
- Significantly reduces carryover contamination
Invitrogen’s SYBR® GreenER™ qPCR SuperMixes and
two-step qRT-PCR kits can be used on virtually any instrument.
Specially optimized kits for ABI and Biorad instruments are available
with premixed ROX or fluorescein reference dye. Click here for more information.
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qPCR & qRT-PCR
Reagent Kits
Invitrogen’s qPCR SuperMixes, one-step qRT-PCR SuperMixes, and two-step
qRT-PCR kits can be used on virtually any instrument. Kits are
available to support sequence-specific detection methods (fluorogenic
probe- or primer-based detection) or with SYBR® Green. Specially
optimized kits for ABI instruments are available with premixed ROX
reference dye.
To find the ideal reagent kit for you, use our qPCR Product
Selection Guide.
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qRT-PCR from Small
Samples: CellsDirect™ qRT-PCR
Obtaining good sensitivity from qPCR can be challenging when your
sample material is limited. Our CellsDirect™ qRT-PCR kits are designed
for maximum sample preservation. With CellsDirect™ qRT-PCR technology
allows you to lyse your cells and proceed directly to qRT-PCR,
eliminating RNA purification steps that can contribute to sample loss
or introduce PCR inhibitors. CellsDirect technology also minimizes
sample processing time and speeds throughput.
CellsDirect qRT-PCR kits are available in 1-step or 2-step formats. Click
here for more information.
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D-LUX™ Select Gene
Expression Assays
D-LUX™ Select Gene Expression Assays combine the best of probe-based
and SYBR® Green-based detection to deliver the most sensitive,
specific, and cost-effective detection method available for
qPCR/qRT-PCR. With the D-LUX™ Gene Expression Assays, you can:
- Quantitate
10 or fewer copies with a dynamic range of up to eight orders of
magnitude
- Perform
melting curve analysis to distinguish genuine amplicons from
primer-dimers and other artifacts
- Multiplex
up to 3 targets per reaction
- Perform
quantitation on any real-time instrument platform
Choose
from over 130,000 pre-designed, in silico validated assays for human,
mouse and rat targets.
Click
here to find an assay for your target of interest.
For an overview of D-LUX™ technology visit www.invitrogen.com/lux
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Contact
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Dr. Debra Ann Nickson
Senior Product Manager
Genomics Invitrogen
debra.nickson@invitrogen.com
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