qPCR INFO PORTAL
With this page and all the presented tools we will help you with to find the right information about qPCR and related topics in Molecular Biology in the literature and in the World Wide Web  =>  qPCR Directory:   A-Z          Share



follow us on twitter => MIQE_qPCR Follow us on twitter - GeneQuan
LinkedIN group - real-time PCR & qPCR
Try a  SCOPUS  search
Try a  PubMed  search
rougly 20 million papers present


Welcome to Stephen Bustin's publishing website
an iTunes eBook series  Definitive qPCR  edited by Stephen Bustin

An exhaustive guide to assay design for quantitative real-time PCR. The book describes the basic concepts important for amplicon selection and primer and probe design. There are step-by-step examples for designing probe-based and SYBR Green assays targeting mRNA and fungal pathogens using several popular design programs. These are then exposed to extensive in silico analysis to identify the optimum amplicon/primer/probe combination. There is a detailed trouble shooting guide, a listing of instruments, reagents and additional information available on the internet, all with hyperlinks. In addition, there are three Keynote presentations summarising the main concepts of standard assay design, multiplex assay design and explaining the rationale behind MIQE, the guidelines for qPCR publication transparency.

=>  Biocompare Product List - PCR    NEW !

=>  Biocompare Product List - real-time PCR    NEW !




Genome Technology Tech Guides Listing

Genotyping/PGx
  • Instrument Guide - Clinical Genotyping
  • Tech Guide - SNP Genotyping
  • Tech Guide - Clinical Genotyping
  • Validating Cancer Biomarkers Tech Guide
  • Genotyping Tech Guide
Microarrays
  • Tech Guide - cDNA Microarrays
  • Tech Guide - Microarray Sample Prep
  • Tech Guide - Microarray Confirmation & Quality Control
  • Tech Guide - Microarray Analysis: Genome-wide Association
  • ChIP-chip Tech Guide
  • Array CGH Tech Guide
Proteomics
  • Tech Guide - Mass Spec Sample Prep
  • Tech Guide - PhosphoProteomics-PTM
  • Tech Guide - Protein-Protein Interactions
  • Tech Guide - High-Throughput Chromatography
  • Tech Guide - Protein Microarrays
  • Tech Guide - MALDI-TOF
PCR
  • Tech Guide - Real Time PCR Volume I
  • Tech Guide - Real Time PCR Volume II
  • Tech Guide - Real Time PCR Volume III
  • Tech Guide - Clinical qPCR (Volume IV)
  • Tech Guide - Real Time PCR for microRNAs
  • Best Practices Guide - Sample Prep
  • Real-time PCR Tech Guide
  • Real-time PCR Tech Guide, vol VII
  • Real-time PCR Tech Guide, vol VIII
RNA Interference
  • Tech Guide - siRNA Design
  • Tech Guide - shRNA Design
Sample Prep
  • DNA Extraction and FFPE Tech Guide
  • Agbio Sample Prep Tech Guide
Sequencing
  • Tech Guide - Sequencing Sample Prep
  • Tech Guide - DNA Sequencing
  • Tech Guide - Sequence Alignment & Assembly
  • Assembly/Alignment for Next-Gen Sequencing Tech Guide



BioMedSearch is an enhanced version of the NIH PubMed search that combines MedLine/PubMed data with data from other sources to make the most comprehensive biomedical literature search available. BioMedSearch also provides advanced account features that allow saved searches, alerts, saving documents to portfolios, commenting on documents and portfolios, and sharing documents with other registered users. Registering for BioMedSearch is free => http://www.biomedsearch.com/


logo Scitable

Scitable is a Social Network for Science

If you have an interest in science, or specifically in genetics, you can always turn to scientific journals and publications for information. But if you're looking for reference material or an opportunity to connect with researchers and scientists in the field, Scitable can help.

Gene Expression and Regulation      Lead Editor:  Laura Hoopes



Scitable is billed as a learning and research tool for people interested in genetics, but under the hood it's much more than just a reference utility. The service offers you the ability to connect with researchers and scientists around the globe and influence the scientific discussion around genetics while also giving you a way to learn more about it.
The first thing you'll want to do at Scitable is sign up for an account. They're free, and give you access to all of the site's features, including the ability to create a profile for yourself, contribute articles and multimedia to the community, and access to the site's over 150 free articles and peer-reviewed papers on topics from genetic engineering to evolutionary biology. The service is aimed primarily at teachers, professors, and their students, but if you have an interest in science, biology, or genetics, don't hesitate to sign up.



DATABASES ON MEDICINE AND MOLECULAR BIOLOGY

from  http://www.meddb.info/index.php.en



What's new at GoPubMed ... ?
  • The first major change is that MeSHPubmed is now included in GoPubMed. Gene Ontology (GO) and Medical Subject Headings (MeSH) are now integrated into one application. This change was requested by many users and enables the joint search in molecular biology and medicine!
  • Novel award winning text mining method for automated recognition of protein names. Proteins are linked to the UniProt database.
  • Using the clipboard functionality the user can collect selected articles from one or more searches and export them to Endnote, BibTeX, Plain text, or XML formats.
  • New export functionalities for each citation or the first 100 search results.
  • Toggle between abstract and snippet view.
  •  HotTopics, an alternative way to get an overview on biomedical literature over the last years, is automatically linked to your query.
  •  A complete new interface. Like it? Drop us a comment: help@transinsight.com ... we appreciate!
How Does It Work?

When people search, they have questions in mind. GoPubMed allows to significantly faster find information needed through the use of background knowledge. =>  GoPubMed
  • retrieves PubMed abstracts for your search query,
  • detects terms from the Gene Ontology (GO) and Medical Subject Headings (MeSH) in the abstracts,
  • displays the subset of the GO and MeSH relevant to the keywords, and
  •  allows you to browse the ontologies and display only papers containing specific GO and MeSH terms.
After performing a search, the resulting abstracts are annotated with your query keywords and GO and MeSH terms. The abstracts are grouped using the GO and MeSH terms, which appear in the text. Now the GO and MeSH hierarchies can be used to systematically explore the search results.

Note that only a subset of all GO and MeSH terms may be relevant to your query. This subset – more frequent terms - is presented on the left hand side. Sorting documents to a highly organised network facilitates the finding of relevant documents significantly.

The hierarchy of content shows the whole GO and MeSH ontologies. GO and MeSH serve as table of contents in order to structure the over 16 million articles of the MEDLINE data base.



New search functions on Google Scholar !


logo GOOGLE
about GOOGLE
logo GOOGLE scholar

Google Scholar enables you to search specifically for scholarly literature, including peer-reviewed papers, theses, books, pre-prints, abstracts and technical reports from all broad areas of research. Use Google Scholar to find articles from a wide variety of academic publishers, professional societies, preprint repositories and universities, as well as scholarly articles available across the web.


Google Scholar: The New Generation of Citation Indexes
by ALIREZA NORUZ, Libri 2005

Google Scholar (http://scholar.google.com) provides a new method of locating potentially relevant articles on a given subject by identifying subsequent articles that cite a previously published article. An important feature of Google Scholar is that researchers can use it to trace interconnections among authors citing articles on the same topic and to determine the frequency with which others cite a specific article, as it has a "cited by" feature. This study begins with an overview of how to use Google Scholar for citation analysis and identifies advanced search techniques not well documented by Google Scholar. This study also compares the citation counts provided by Web of Science and Google Scholar for articles in the field of "Webometrics." It makes several suggestions for improving Google Scholar. Finally, it concludes that Google Scholar provides a free alternative or complement to other citation indexes.

Can't find the right info or publication on my sites ?



Try a PubMed search !


  searching for qPCR
  searching for quantitative PCR

  searching for real-time PCR   searching for expression profiling

  searching for reference gene
  searching for PCR & normalisation



Keywords     Topics     Search

DiscoveR8.com provides the scientific community with a place to share and promote your discoveries. Our users can both rate the general quality and overall relevance of each posted article. By recommending your own or others' papers on DiscoveR8, you not only grant them wider exposure to the internet community at large but also potentially increase its professional impact. DiscvoeR8.com has just entered its beta test cycle. We would like to invite you to the beta test. By participating in the beta test period of a site like DiscoveR8, you would set precedents for the tone, decorum, range of topics and level of intellectual depth of the discussion. We welcome any contribution that you might see fit to make.




=>  VIEW NEWS BY SUBJECT



GEN News Highlights

PCR
All news related to enzymatic DNA replication.

Gene Silencing
What's new in transcriptional and  post-transcriptional regulation.

Informatics
The latest from the field of computational biology.

Educational Resources:  Web based Dictionaries & Glossaries


 Newsgroups & e-Groups
 

BioTechniques® Molecular Biology Techniques Forums
Welcome to the BioTechniques® Molecular Biology Forums, a science-based bulletin board for techniques, tips, and questions concerning molecular biology, cell biology, microscopy, and bioinformatics.
MolecularBiology.forums.biotechniques.com



logo MultiD
qPCRforum.com : Discuss GenEx and qPCR

Welcome to qPCRforum
- Forum for discussion about GenEx and qPCR (quantitative real-time PCR)




qPCR Listserver ( biggest qPCR group ! )




Scientist Solutions - Your International Life Science Form
By Scientists.....for Scientists

The ScientistSolutions board provides a discussion forum for professionals in the life sciences, where researchers can ask and answer questions, exchange protocols, and post jobs.  www.scientistsolutions.com








http://www.nature.com/protometh/index.html

Nature Protocols is an online resource for protocols, including authoritative, peer-reviewed 'Nature Protocols' and an interactive 'Protocols Network'. The two create a dynamic forum for scientists to upload and comment on protocols.

  • Upload your own lab protocols for further feedback and refinement
  • Comment on published Nature Protocols, or protocols submitted by other researchers
  • Discuss techniques in our discussion forum
  • Read latest protocols on the network




Bio Forum Methods and Products Discussion:




http://www.protocol-online.org/

Popular Categories

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BioTechniques' Protocol Guide Offers Solutions for Science.

BioTechniques®' Protocol Guide is a collection of detailed, optimized procedures/protocols for use in the lab. Organized by category, BioTechniques' Protocol Guide contains specific applications of commercial products in a wide range of areas. Even better, protocols are updated quarterly to provide you with the very latest product information. 
With BioTechniques®' Protocol Guide, you have access to detailed product information in a downloadable PDF format-which can be easily added to a notebook for ready access. Each protocol is accompanied by a link to the company web site and an e-mail link to contact the company directly.
http://www.biotechniques.com/default.asp?page=protocol

High Resolution Melt (HRM) by Corbett Life Science
BioTechniques Protocol Guide: p 67 (Dec 2006)  view protocol

Complete Real-Time PCR Solutions by Applied Biosystems
BioTechniques Protocol Guide: p 71 (Dec 2006)  view protocol

Normalization Methods for qPCR by Bio-Rad Laboratories
BioTechniques Protocol Guide: p 63 (Nov 2007)  
view protocol




Nucleic Acids Research Methods

Each of the collections on this page is a category-specific archive of Methods published in NAR from 1999 to the present. The numbers in parentheses show the number of articles currently in each collection.

©  editor@gene-quantification.info