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keynote speaker: |
preliminary
title |
The Pioneer in PCR |
Kary B. Mullis
1993
Nobel Prize in Chemistry
In 1993, the Royal Swedish Academy of Sciences awarded the Nobel Prize
in Chemistry for work on DNA-based chemistry methods, with one-half of
the prize going to Kary Mullis for his invention of the polymerase
chain reaction (PCR) method. His work, the academy said, had "hastened
the rapid development of genetic engineering" and "greatly stimulated
biochemical research and opened the way for new applications in
medicine and biology."
The academy added: "The applications of Mullis' PCR method are already
many. It is for example possible using simple equipment to multiply a
given DNA segment from a complicated genetic material millions of times
in a few hours, which is of very great significance for biochemical and
genetic research. The method offers new possibilities particularly in
medical diagnostics, and is used, for example, for discovering HIV
virus or faulty genes in hereditary diseases."
|
25th Anniversary of PCR
|
Molecular
Diagnostics
qPCR NOS Session
Normalization & Optimization &
Standardization
|
Stephen A. Bustin
Professor of Molecular Science, Institute of Cell and Molecular
Science, Queen Mary's School of Medicine and Dentistry, University of
London, UK
Stephen Bustin obtained his PhD from Trinity College, University of
Dublin in molecular genetics in 1983. Since 1989 he has worked at the
Royal London Hospital, aiming to apply his research in a more direct,
practical setting. Following promotion to Senior Lecturer (1995) and
Reader in Molecular Medicine (2002) he was awarded a personal chair by
the University of London in 2004. He was appointed as a visiting
Professor of Molecular Biology by the University of Middlesex in 2006.
His main area of research is into bowel-associated pathologies,
especially colorectal cancer and, more recently, Clostridium
difficile-associated disease. He has a special interest in molecular
technologies and his laboratory operates at the forefront of
technological development in nucleic acid quantification, where he is
an internationally acknowledged leader. He has published numerous
peer-reviewed papers and reviews and is the editor of the “A-Z of
quantitative PCR”, the leading textbook for this technology. He is on
the editorial boards of several journals and has given numerous
presentations at scientific conferences around the world. He has
organized and co-organised many qPCR meetings in the UK, Europe and the
US.
|
A new qPCR assay for
the detection of Clostridium difficile
HOT TOPIC
MIQE- guidelines for
publication of qPCR data
|
High
Throughput
|
Ken
Livak
Senior
Scientific Fellow, Fluidigm
Corporation,San Francisco,
CA, US
Dr. Livak has recently joined Fluidigm Corporation as Senior Scientific
Fellow and is charged with pioneering novel assays on Fluidigm’s
microfluidic platform. During his 13 years at Applied Biosystems,
he
concentrated on improving the robustness and versatility of using
fluorogenic (TaqMan) probes in the 5’ nuclease PCR assay.
Accomplishments include the discovery of a general method for probe
design, application of fluorogenic probes to allelic discrimination,
and elucidation of how to use real-time PCR data for quantification of
nucleic acids. As Vice President, Science, he reviewed and
coordinated
scientific efforts across all the instrument platforms developed by
Applied Biosystems in order to foster technology innovation in the
broad area of life science discovery.
|
Moving
from qPCR Assays to qPCR Arrays. |
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|
invited
academic speaker:
|
preliminary
title |
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Molecular
Diagnostics
RNAi - microRNA -
siRNA Applications
|
Jo Vandesompele
Center for Medical Genetics Ghent, Ghent University Hospital, Ghent, Belgium
|
Multi-gene
signature for cancer patient survival.
Normalization of miRNA data.
|
Molecular
Diagnostics
|
Andreas Nitsche
Robert Koch Institut ZBS 1, Berlin, Germany
|
Real-time PCR
based Diagnostic of highly pathogenic Viruses. |
Single-cell
qPCR |
Anders
Stahlberg
Stem Cell
Center
Lund University, Lund, Sweden
|
Technical
aspects of mRNA quantification in single cells using RT-qPCR. |
RNAi - microRNA -
siRNA Applications |
Greg
Shipley
Director, Quantitative Genomics Core, Laboratory, The University of
Texas Health Science Centre- Houston, TX,
US
|
A
Multi-assay Approach to the Study of Cellular Toxicity. |
Bioinformatics |
Mikael Kubista
Professor
of Biotechnology, TATAA Biocenter Sweden,
MultiD
Analyses
AB, Göteborg, Sweden
|
Gene Expression
Profiling with qPCR. |
Copy
Number Variation
|
Jan Helemanns
Center for Medical Genetics Ghent, Ghent University Hospital, Ghent, Belgium
Biogazelle NV, Kruishoutemstraat 57, Zulte, Belgium
|
Accurate
and objective copy number profiling. |
Molecular
Diagnostics |
Dimitrios Frangoulidis
Institut für Mikrobiologie der Bundeswehr, Munich,
Germany
|
Real-time
PCR of bioterrorism agents. |
Molecular
Diagnostics |
Olfert Landt
TIB MOLBIOL Syntheselabor GmbH, Eresburgstrasse, Berlin, Germany
|
Probe Based Detection
of Genetic Variations - Screening and in-vitro Diagnostics.
|
Pre-PCR
optimisation |
Michael W. Pfaffl
Reader in
Physiology, Center of Life Science, Weihenstephan, Technical University
of Munich, Freising, Germany
|
mRNA
& microRNA integrity - the key to success. |
Single-cell
qPCR |
Birgit Liss
Institut für Allgemeine Physiologie, Universität Ulm, Germany
|
qPCR
of indicidual dopamine neurons form mouse models and human post mortem
brain sections. |
Molecular
Diagnostics |
Jim
Huggett
Centre for Infectious Diseases & International Health, University
College London, UK
|
The
use of nucleic acid amplification tests for research and diagnosis in
the developing world.
|
RNAi - microRNA - siRNA Applications
|
Christian Thirion
Managing Director, SIRION GmbH, Planegg/Martinsried, Germany
|
An
inflammatory microRNA signature in muscle cells - a comparative study
of cellular models and technological platforms.
|
Molecular
Diagnostics |
Udo Reischl
Institut für Medizinische Mikrobiologie und Hygiene
- Universität
Regensburg, Germany
|
Current applications
of real-time PCR technology in diagnostic bacteriology.
|
Bioinformatics |
Ales Tichopad
Labonnet LTD / Technical University Munich, Physiology Weihenstephan
|
Adequate experiment
design as the first important step in obtaining valid biological
inference with qPCR technique.
|
Bioinformatics
Databanking
|
Filip Pattyn
Center for Medical Genetics Ghent, Ghent University Hospital, Ghent, Belgium
|
New
RTPrimerDB. |
Molecular
Diagnostics |
Pamela
Pinzani
Clinical Biochemistry
Unit, Department
of Clinical Physiopathology, University of Florence, Florence, Italy
|
Circulating nucleic
acids in melanoma diagnosis.
|
Bioinformatics |
Anders Bergkvist
Senior Scientist, Head of Business Development MultiD Analyses AB,
Sweden
|
Data
analysis for gene quantification and expression profiling using GenEx.
|
RNAi - microRNA - siRNA Applications
|
Mirco Castoldi
EMBL Heidelberg, Heidelberg, Germany
|
miQPCR
- a novel approach for expression profiling of mature microRNAs. |
Molecular
Diagnostics |
Hans
Nitschko
Max
von Pettenkofer-Institut, Ludwig-Maximilians-University Munich,
Department of Virology, Germany
|
Multiplex-PCR
in clinical virology - benefits and limitations. |
Molecular
Diagnostics
in agricultural and veterinary Science
|
Irmgard
Riedmaier
Physiology
Weihenstephan, Center of Life Science, Technical University
of Munich, Freising, Germany
|
The use of
transcriptomics for biomarker development to trace anabolic hormone
functions.
|
Molecular
Diagnostics
in agricultural and veterinary Science
|
Huber Ingrid
Bavarian Health and Food Safety Authority, Germany
|
Rapid detection and
differentiation of Campylobacter jejuni, C. coli and C. lari in food
samples using a quadruplex real-time PCR assay.
|
Bioinformatics |
Jochen Blom
Center for Biotechnology, Bielefeld University, Germany
|
CAmpER
- An open analysis framework for real-time PCR data using single sample
amplification efficiency calculation.
|
Molecular
Diagnostics |
Kim De Leeneer
Center for Medical Genetics Ghent (CMGG),
Ghent University
Hospital, Medical Research Building (MRB), De Pintelaan 185, Ghent,
Belgium
invited by GOLD SPONSOR Bio-Rad
Laboratories, Hercules, US
|
Applications of
HRM Curve Analysis: Strengths and Pitfalls.
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industrial R&D speaker:
|
preliminary
title |
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LEAD
SPONSOR:
|
|
High
Throughput |
Thomas Froehlich
Director R&D, Roche Applied Science, Mannheim,
Germany
|
LightCycler 1536
System - Introducing the next Level of High-Throughput PCR.
|
qPCR NOS Session
|
Ralf
Mauritz
Director R&D,
Roche Applied Science, Mannheim, Germany
|
RealTime ready –
Functionally Tested Assays for Gene Expression Analysis on the
LightCycler Platform.
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GOLD
SPONSORS: |
|
High
Throughput |
Ken
Livak
Senior
Scientific Fellow, Fluidigm
Corporation,San Francisco,
CA, US
|
Moving
from qPCR Assays to qPCR Arrays. |
RNAi - microRNA - siRNA Applications
|
Jason
Halsey
VP of Molecular Biology Systems R&D, Applied
Biosystems, R&D, US
|
Discovery
and Validation of Novel Human MicroRNA Genes by SOLiD and
TaqMan. |
Molecular
Diagnostics |
Debra Nickson
Regional Business Manager, Gene Expression Profiling, Invitrogen,
United Kingdom
|
Gene Scanning by
High-Resolution Melt (HRM) using EXPRESS SYBR GreenER qPCR SuperMix.
|
qPCR NOS Session
|
Steffen
Müller
Agilent Technologies
Inc, Stevens Creek Blvd, Santa Clara , CA, US
|
Importance of
experimental design and sample QC for robust and meaningful QPCR
results.
|
RNAi - microRNA - siRNA Applications
|
Martin Kreutz
Scientist R&D – miRNA Research, QIAGEN GmbH, Hilden, Germany
|
Quantification and
Functional Analysis of miRNA in Mammalian Cells.
|
High
Throughput |
Ben Sowers
Biosearch Technologies, Novato,
CA, US
|
Oligo Design Across
the Mouse Genome.
|
qPCR NOS Session
|
Ian
Kavanagh
Abgene,
Thermo Fisher Scientific, UK
|
Increasing qPCR
throughput: simple steps to speed up results whilst minimising variance.
|
Molecular
Diagnostics |
Jim Thorn
Product
Manager Genetic Analysis, Beckman
Coulter GmbH - Diagnostics, Europark Fichtenhain, Krefeld, Germany
|
A Novel Multiplex,
Quantitative Gene Expression Approach for Cancer Biomarker Research.
|
RNAi - microRNA -
siRNA Applications |
Ditte Andreasen
Exiqon, R&D, Vedbaek, Denmark
|
Highly
sensitive and specific LNA enhanced real-time PCR for microRNA
expression analysis.
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SESSION
SPONSORS:
|
|
25th Anniversary of
PCR |
Nobel
Prize
Laureate Kary Mullis
sponsored by
Biosearch Technologies, Novato,
CA, US
|
25th Anniversary of
PCR. |
qPCR NOS Session
|
Daniel J. Shaffer
Vice President and Founder, Bar Harbor BioTechnology, Inc., USA
|
Highly Accurate
Quantitative Gene Expression Analysis without Use of pre-defined
Normalizer Genes using Pattern Recognition Analysis.
|
Diagnostics
& Molecular Markers
|
Rob
Powell
PrimerDesign,
Millbrook Technology Campus, Southampton Hants, UK
|
High resolution melt
analysis for low resolution machines.
|
High throughput qPCR |
Natasha
Paul
TriLink BioTechnologies, San Diego, CA, USA
|
The
Next Generation in Hot Start PCR - CleanAmp Primers and dNTPs.
|
Single-cell qPCR |
Jim White
Business Development, BioTrove Inc., USA
Alpha Metrix Biotech, Rödermark, Germany
|
Measurement of Gene
Expression by Massively Parallel Nanoliter real-time PCR.
|
RNAi - microRNA -
siRNA Applications |
Carola Wagner
IMGM Laboratories GmbH, Martinsried, Germany |
microRNAs
- developing new tools for diagnostics - Join forces with IMGM
Laboratories to make your miRNA project a success.
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