of stable housekeeping genes, differentially regulated target genes and
housekeeping genes, differentially regulated target genes
stability of standard gene expression is an elementary prerequisite for
internal standardisation of target gene expression data and many so
called housekeeping genes with assumed
stable expression can exhibit either upor down-regulation under some
experimental conditions. The developed, and
herein presented, software called BestKeeper determines the best suited
standards, out of ten candidates,
and combines them into an index. The index can be compared with further
ten target genes to decide, whether
they are differentially expressed under an applied treatment. All data
processing is based on crossing points. The
BestKeeper software tool was validated on four housekeeping genes and
10 members of the somatotropic axis
differentially expressed in bovine corpora lutea total RNA. The
BestKeeper application and necessary information
about data processing and handling can be downloaded on here.
The paper has been frequently cited by other researchers:
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Important note - Computer configurations
order to be able to run the Relative Expression Software Tool
Tools => Macro => Security =>
and to set the 'Security Level' to 'Medium'
shown in the following two screen shot pictures.
Page 1: Introduction & Input - real-time PCR Efficiency
Page 2: Input - of Housekeeping Gene CP Data
Page 3: Input - of Target Gene CP Data
Page 4: Output - HKG CP Data & Repeated Pair-wise Correlation Analysis of HKG
Page 5: Output - Repeated Pair-wise Regression Analysis of HKG
Page 6: Output - HKG CP Data & Repeated Pair-wise Correlation Analysis of HKG
Page 7: Output - Repeated Pair-wise Correlation and Regression Analysis of TG
Page 8: Output - Sample Intergrity
Page 9: Output - Graph of HKG CPs
Page 10: Output - Graph of TG CPs